Study of Relationship between Serum TREM-1,TLR4 and IL-37 Levels and T Lymphocyte Subsets in Patients with Allergic Rhinitis
Objective:To investigate the relationship between serum levels of triggering receptor expressed on myeloid cells-1(TREM-1),Toll-like receptor 4(TLR4)and interleukin-37(IL-37)and T lymphocyte subsets in patients with allergic rhinitis(AR).Methods:100 AR patients who were admitted to Huaian First Affiliated Hospital of Nanjing Medical University and Northern Jiangsu People's Hospital of Jiangsu Province from March 2021 to March 2023 were selected as AR group,and 100 healthy volunteers who underwent physical examination during the same period were selected as control group,AR patients were divided into moderate to severe AR group(63 cases)and mild AR group(37 cases)according to the severity of the disease.Serum TREM-1,TLR4,IL-37 levels and peripheral blood T lymphocyte subsets were detected by enzyme-linked immunosorbent assay and flow cytometry.The correlation between serum TREM-1,TLR4,IL-37 levels and peripheral blood T lymphocyte subsets in AR patients were analyzed by Pearson/Spearman.The diag-nostic value of serum TREM-1,TLR4 and IL-37 levels for AR were analyzed by drawn receiver operating characteristic(ROC)curve.Results:Compared with control group,serum TREM-1,TLR4 and peripheral blood CD8+ratio were increased,serum IL-37 and periph-eral blood CD3+,CD4+,CD4+/CD8+were decreased in AR group(P＜0.05).Compared with mild AR group,serum TREM-1,TLR4 and peripheral blood CD8+ratio were increased,serum IL-37 and peripheral blood CD3+,CD4+,CD4+/CD8+were decreased in moderate and severe AR group(P＜0.05).Pearson/Spearman correlation analysis showed that,serum TREM-1 and TLR4 levels in AR patients were neg-atively correlated with peripheral blood CD3+,CD4+,CD4+/CD8+,and positively correlated with peripheral blood CD8+ratio(P＜0.05).Serum IL-37 level was positively correlated with peripheral blood CD3+,CD4+,CD4+/CD8+,and negatively correlated with peripheral blood CD8+ratio(P＜0.05).ROC curve analysis showed that,the area under the curve of serum TREM-1,TLR4 and IL-37 levels in the di-agnosis of AR was 0.960,which was greater than 0.851,0.857 and 0.844 predicted by serum TREM-1,TLR4 and IL-37 levels alone.Conclusion:The levels of serum TREM-1 and TLR4 in AR patients are increase,and the level of IL-37 is decrease,which is closely re-late to the abnormality of T lymphocyte subsets,the detection of serum TREM-1,TLR4 and IL-37 levels is helpful to evaluate the patho-genesis and progression of AR.
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