目的:探讨人乳头瘤病毒(HPV)感染宫颈癌(CC)组织溶血磷脂酰胆碱酰基转移酶1(LPCAT1)、细胞因子信号传导抑制因子2(SOCS2)的表达情况及其临床价值。方法:选取2018年1月～2020年5月苏州大学附属第二医院妇科收治的153例HPV阳性CC患者和60例HPV阴性CC患者，收集术中HPV阳性CC组织及HPV阴性CC组织与对应癌旁组织，采用免疫组化法检测LPCAT1、SOCS2表达。分析HPV阳性CC组织LPCAT1、SOCS2表达与临床病理特征的关系。根据HPV阳性CC组织LPCAT1、SOCS2阳性/阴性表达分为LPCAT1、SOCS2阳性/阴性表达组。Kaplan-Meier法绘制LPCAT1、SOCS2阳性/阴性表达HPV感染CC患者3年总生存期(OS)和无病生存期(DFS)曲线，Cox回归分析影响HPV感染CC患者预后的因素。结果:癌旁组织、HPV阴性CC组织、HPV阳性CC组织LPCAT1阳性表达率依次升高，SOCS2阳性表达率依次降低(P＜0。05)。不同分化程度、间质浸润深度、国际妇产科联盟(FIGO)分期、淋巴结转移的HPV感染CC组织LPCAT1、SOCS2阳性表达率有差异(P＜0。05)。随访 3 年，153 例 HPV 感染 CC 患者 OS 为 78。43％(120/153)、DFS 为 61。44％(94/153)。Kaplan-Meier 生存曲线显示，LPCAT1阳性表达组OS、DFS低于LPCAT1阴性表达组，SOCS2阳性表达组OS、DFS高于SOCS2阴性表达组(P＜0。05)。多因素Cox回归分析显示，低分化、间质浸润深度≥3 mm、FIGO分期Ⅲ期、淋巴结转移、LPCAT1阳性为影响HPV感染CC患者预后的独立危险因素，SOCS2阳性为独立保护因素(P＜0。05)。结论:HPV阳性CC组织LPCAT1高表达，SOCS2低表达，与分化程度、间质浸润深度、FIGO分期、淋巴结转移和预后有关，可能成为HPV阳性CC患者预后评估指标。
Study on the Expression and Clinical Value of LPCAT1 and SOCS2 in HPV Infection Cervical Cancer Tissues
Objective:To investigate expression and clinical value of lysophosphatidylcholine acyltransferase 1(LPCAT1),and suppressor of cytokine signaling 2(SOCS2)in human papillomavirus(HPV)infection cervical cancer(CC)tissues.Methods:153 HPV positive CC patients and 60 HPV negative CC patients who were admitted to the Gynecology Department of the Second Affiliated Hospi-tal of Soochow University from January 2018 to May 2020 were selected,HPV positive CC tissues and HPV negative CC tissues and paracancerous tissues were collected during operation,the expression of LPCAT1 and SOCS2 was detected by immunohistochemistry.The relationship between the expression of LPCAT1 and SOCS2 in HPV positive CC tissues and clinicopathological features was ana-lyzed.Patients were divided into LPCAT1 and SOCS2 positive/negative expression groups according to the positive/negative expression of LPCAT1 and SOCS2 in HPV positive CC tissues.The 3-year overall survival(OS)and disease-free survival(DFS)curves of HPV infection CC patients with positive/negative expression of LPCAT1 and SOCS2 were drawn by Kaplan-Meier method,the factors affecting the prognosis of HPV infection CC patients were analyzed by Cox regression analysis.Results:The positive expression rate of LPCAT1 in paracancerous tissues,HPV negative CC tissues and HPV positive CC tissues increased in turn,and the positive expression rate of SOCS2 decreased in turn(P＜0.05).The positive expression rates of LPCAT1 and SOCS2 in HPV infected CC tissues with different de-grees of differentiation,depth of interstitial infiltration,International Federation of Gynecology and Obstetrics(FIGO)stage and lymph node metastasis were different(P＜0.05).After 3 years of follow-up,the OS and DFS of 153 CC patients with HPV infection were 78.43％(120/153)and 61.44％(94/153).Kaplan-Meier survival curve showed that,OS and DFS in LPCAT1 positive expression group were lower than those in LPCAT1 negative expression group,and OS and DFS in SOCS2 positive expression group were higher than those in SOCS2 negative expression group(P＜0.05).Multivariate Cox regression analysis showed that,the independent risk factors affecting the prognosis of HPV infection CC patients were poor differentiation,depth of interstitial infiltration ≥3 mm,FIGO stage Ⅲ,lymph node metastasis and LPCAT1 positive,and the independent protective factor was SOCS2 positive(P＜0.05).Conclusion:High expression of LPCAT1 and low expression of SOCS2 in HPV positive CC tissues are relate to the degrees of differentiation,depth of interstitial infiltra-tion,FIGO stage,lymph node metastasis and prognosis,which may be a prognostic indicator for HPV positive CC patients.
Human papillomavirusCervical cancerLPCAT1SOCS2Clinicopathological featuresPrognosis
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万方数据
