Biochemical and Plasma Metabolomics of Quercetin Feeding Rats with Type Ⅰ Diabetes
Objective:To investigate the therapeutic effects of feeding quercetin on streptozocin-induced type 1 diabetic rats and the associated metabolic pathway changes.Methods:Type 1 diabetic rat models were established by intraperitoneal injection of streptozotocin at a dose of 60 mg/kg.Rats were randomly divided into the model(T1DM)group,quercetin low-dose treatment(T1DM+QUE(25))group,and quercetin high-dose treatment(T1DM+QUE(50))group.The treatment groups were given quercetin at the corresponding concentrations(mg/kg),while the normal control group(Normal)and the T1DM group were given an equivalent volume of blank solvent.After 8 weeks of continuous feeding,plasma samples were collected for the detection of acetoacetic acid,glycosylated hemoglobin,alanine aminotransferase,and blood lipids.Muscle tissue samples were collected to measure glycogen synthase levels.Plasma metabolomics analysis was performed using LC-MS/MS.Results:Compared with that in the T1DM group,acetoacetic acid,aspartate aminotransferase in plasma in the T1DM+QUE(50)group decrease(P＜0.05),while total antioxidant capacity levels in lipoprotein lipase increased(P＜0.05),and their levels were closer to Normal group than T1DM group.The levels of glycosylated hemoglobin,alanine aminotransferase,low-density lipoprotein and total cholesterol were also improved,but not significantly.The expression of glycogen synthase protein in muscle was also significantly improved(P＜0.05).Metabolomics analysis results showed that quercetin feeding mainly interfered with sphingolipid metabolism and synthesis,steroid hormone biosynthesis,retinol metabolism,arachidonic acid metabolism and other metabolic pathways.Conclusions:Feeding quercetin can improve lipid and cholesterol metabolism in type 1 diabetic rats,alleviate liver damage,and achieve certain therapeutic effects by influencing lipid metabolism and modulating inflammation-related pathways.
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