Study on The Role of AQP1 in Organ Edema in Rats with Adriamycin Nephropathy
Objective:To investigate whether adriamycin nephropathy causes edema in organs other than kidney and the role of AQP1 expression change in organ edema.Method:Twenty SD rats were randomly divided into control group and model group.The model group was injected with adriamycin(6.0 mg/kg)via tail vein,and the control group was injected with the same amount of normal saline.The rats were sacrificed after 6 weeks,and the body weight and the weights of heart,liver,spleen,and lung were recorded.The pathology of the heart,liver,spleen,and lung were observed by HE staining.The distribution of AQP1 in heart,liver,spleen,and lung was detected by IHC method.And the protein and mRNA expression of AQP1 in each organ were detected by Western blot and RT-PCR.Result:Compared to the control group,the urinary protein of adriamycin nephropathy rats increased significantly(P＜0.01),the body weight decreased significantly(P＜0.05),and the lung coefficient increased significantly(P＜0.05).Whereas,there were no significant differences in heart,liver,and spleen weights between control and model group.The heart,liver and lung showed different degrees of edema and pathological damage,and the spleen showed insufficient blood filling.The immunohistochemistry results showed that AQP1 was distributed in hepatic cholangiocytes,cardiac myocyte membranes,and microvascular endothelial cells,spleen erythrocyte,and pulmonary capillary endothelial cells.The results of Western blot and RT-PCR showed that the expression of AQP1 protein and mRNA in the heart of the model group was significantly increased(P＜0.05).And the expression of AQP1 protein in the liver was increased,but there was no statistically significant difference.Conclusion:Adriamycin nephropathy can cause heart,liver,and lung edema appear different degrees of damage,and up-regulate the expression of AQP1 in the heart to promote the formation of myocardial edema.
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