The Mechanism of LncRNA LUCAT1 Regulating miR-375/HMGB1 Axis on Insulin Resistance in Polycystic Ovarian Syndrome Rats
Objective:To investigate the molecular mechanism of long-chain non-coding RNA(LncRNA)LUCAT1 regulating miR-375/high mobility group protein-1(HMGB1)axis on insulin resistance in polycystic ovary syndrome(PCOS)rats,so as to provide new targets for clinical intervention of disease.Methods:Twenty 3-week-old(average weight 50 g)female SD rats were selected and randomly divided into control group and model group,with 10 rats in each group.The model group replicated PCOS model by subcutaneous injection of dehydroepiandrosterone(DHEA).qRT-PCR was used to detect the expression levels of LncRNA LUCAT1 and miR-375 in ovarian tissues,Western blot was used to detect HMGB1 protein,routine biochemical methods were used to detect serum fasting blood glucose(FBG)and fasting insulin(FINS),and insulin resistance index(HOMA-IR)was calculated.Subsequently,ovarian granulosa cells(GCs)were isolated and immunofluorescence was used to detect follicle stimulating hormone receptors(FSHR)for cell identification.Si-LUCAT1 and si-NC in vitro were constructed then transfected into GCs.After 48 hours of cultivation,the expression levels of LUCAT1,miR-375,and HMGB1 were detected.Results:Compared with the control group of rats,the expression levels of LncRNA LUCAT1 and HMGB1 protein in the ovarian tissues of the model group were significantly higher,while the expression level of miR-375 was significantly lower(P＜0.05);the levels of serum FBG and FINS,as well as HOMA-IR value,were significantly higher,too(P＜0.05).Compared with the si-NC group of GCs cells,the expression levels of LUCAT1 and HMGB1 in the si-LUCAT1 group were signifiicantly less,while the expression level of miR-375 was more,too(P＜0.05).Conclusion:LncRNA LUCAT1 may participate in the occurrence of PCOS and insulin resistance by regulating the biological functions of miR-375/HMGB1 axis.
Polycystic ovary syndromeInsulin resistanceLong chain non-coding RNALUCAT1MiR-375High mobility group protein-1
NETL
NSTL
万方数据
