Effects of Different Fixative Fluids on the Quality of Paraffin Sections in Immune Organs of Guinea Pigs
Objective:To investigate the fixation effect of different fixatives on immune organs of SPF grade guinea pigs,and pro-vide reference for screening fixatives suitable for spleen,lymph nodes and bone marrow.Methods:Twenty-one 300-350 g SPF male guinea pigs were randomly divided into 7 groups with 3 in each group.Spleen,mesenteric lymph nodes and iliac bone marrow were ex-tracted.Each group was fixed with 4％polyformaldehyde solution,10％neutral formalin solution,Bouin's solution,Carnoy solution,Davidson's solution,Zenker solution and Helly solution for 48 h,respectively.Before fixation,iliac bone marrow was immersed in EDTA decalcification solution(pH7.2)and placed in a incubator at 37 ℃ for decalcification.By making paraffin sections and staining with HE,the effects of 7 fixative fluids on section quality were compared from the aspects of section cracking degree,cell morphology and staining effect.Results:The results showed that the white pulp and red pulp of spleen after 10％neutral formalin solution were clearly colored,no cracks appeared,and the morphology of lymphocytes was easy to distinguish.After fixation with Carnoy solution,the bright and dark areas of the germinal center of the lymph nodes were distinct,the red and blue staining was moderate,and the lymphocyte morphology was clear.After fixation with 4％paraformaldehyde solution,bone marrow staining was moderate and clearly distinguishable.Conclu-sions:For tissue fixation at room temperature for 48 h,after HE staining,it is recommended to use 10％neutral formalin solution to fix the spleen,Camoy solution to fix the lymph nodes,and 4％paraformaldehyde solution to fix the bone marrow,and Bouin's solution to fix the bone marrow if decalcification while fixation is considered.
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