Analysis of the Mechanism of Black Raspberry Polyphenols on Gastric Cancer Rats by Regulating STAT3/Survivin Signaling Pathway
Objective:The mechanism of action of black raspberry polyphenols on gastric cancer rats by regulating STAT3/Sur-vivin signaling pathway was analyzed.Methods:A total of 40 male healthy SD rats were selected,10 of which were taken as the blank control group,and the other 30 were used to prepare the gastric cancer rat model.After successful modeling,they were divided into the model group and the black raspberry polyphenols(low and high dose)group,with 10 rats in each group.The rats in the black raspberry polyphenol(low and high dose)group were fed with 5％and 10％black raspberry polyphenol feed,and the rats in the blank control group and the model group were fed with normal feed.After 8 weeks of continuous intervention,the body weight and tumor volume of the rats were measured.The expression levels of STAT3 and Survivin protein were detected by Western blot.Histopathological and in-flammatory factor levels were detected by enzyme-linked immunosorbent assay(ELISA).Results:The body weight and tumor volume of the black raspberry polyphenol(low and high dose)groups were lower than those of the model group,and the high dose group was sig-nificantly lower than the low dose group(P＜0.05).Compared with the expression levels of STAT3 and Survivin protein,the black rasp-berry polyphenol(low and high dose)groups were lower than the model group,and the high dose group was significantly lower than the low dose group(P＜0.05).The levels of motilin(MTL),pepsinogen Ⅰ(PGⅠ)and gastrin(GAS)in black raspberry polyphenol(low and high dose)groups were significantly higher than those in model group,and those in high dose group were significantly higher than those in low dose group(P＜0.05).The levels of interleukin-1(IL-1),interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in black raspber-ry polyphenol(low and high dose)groups were significantly higher than those in model group,and those in high dose group were higher than those in low dose group(P＜0.05).Conclusion:Black raspberry polyphenols can inhibit the growth of gastric cancer in rats by regu-lating STAT3/Survivin signaling pathway,effectively regulate gastrointestinal hormones,improve gastric function,and interfere with the progression of gastric cancer by affecting the level of inflammatory factors.
Gastric cancer ratsBlack raspberry polyphenolsTAT3/Survivin signaling pathwayMechanism of action
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