Correlation Analysis between Changes in Intestinal Flora and Macrophage Polarization in Mice with Colorectal Cancer
Objective:Correlation between changes in intestinal flora and macrophage polarization in colorectal cancer mice ana-lyzed by macrogene sequencing.Methods:3-week-old APCmin/+mice and wild-type C57/6J mice were divided into colorectal cancer group(CRC group,n=8)and normal control group(NC group,n=7),which were given high-fat diet fed for 8 weeks,collect feces and analysis of intestinal flora changes by macrogenetic sequencing,Immunohistochemical(IHC)detect M2 macrophage polarization in tu-mor tissue.Results:HE pathology shows successful modeling in CRC group mice,significantly more tumors than NC group(P＜0.05).The α-diversity showed that CRC group flora diversity higher than NC group,flora richness no significant difference;diversity showed clear separation of the flora between two groups.The Venn diagram demonstrated a total of 1,620 species between the two groups,with 109 species endemic to the NC group and 191 species endemic to the CRC group respectively.At the phylum level,the CRC and NC groups were dominated by the Firmicutes(74.04％vs.87.56％)and the Bacteroidota(20.33％vs.6.42％);at the species level,Metastats analysis showed that the species of Clostridioides difficile_A,Duncaniella freteri,Parabacteroides merdae,and Odoribacter splanchnicus were significantly different between the two groups(P＜0.05);KEGG functional analysis shows that in the CRC group Ribosome,Glycol-ysis,Gluconeogenesis,Oxidative phosphorylation pathway were significantly enriched in the CRC group;Neuroactiveligand receptorin-teraction,Human T_cellleukemiavirus1 infection,Cholesterol metabolism pathway were significantly enriched in the NC group;immuno-histochemical analyses showed that M2 macrophage CD206 content was higher in the CRC,Pearson correlation analyses showed that abundance of Odoribacter splanchnicus was positively correlated to the CD206(r=0.7799).Conclusion:Significant changes and func-tional differences in the intestinal flora of colorectal cancer mice,there may be a correlation between the differential flora and macrophage polarization.
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