Effect of Shikonin Regulating IL-6/STAT3 Signaling Pathway on Pulp Tissue Injury in Pulpitis Rats
Objective:To investigate the effect and mechanism of shikonin(SHI)on pulp tissue injury in pulpitis rats by regulat-ing interleukin(IL)-6/signal transducer and activator of transcription 3(STAT3)signaling pathway.Methods:The pulpitis rat model was established.The experiment was divided into control group(Control group),model group(Model group),SHI low,medium and high dose group(SHI-L,SHI-M,SHI-H group,0.125 mg/kg/d,0.25 mg/kg/d,0.5 mg/kg/d SHI),SHI high dose+STAT3 agonist Colivelin group(SHI-H+Colivelin group,0.5 mg/kg/d SHI+1mg/kg/d Colivelin),18 rats in each group.The general behavior changes of rats were observed.The levels of serum IL-6,IL-1β,tumor necrosis factor-α(TNF-α),CXC chemokine ligand 10(CXCL10)and vascular en-dothelial growth factor(VEGF)were detected by enzyme-linked immunosorbent assay(ELISA).The pathological changes of dental pulp tissue were observed by hematoxylin-eosin(HE)staining;the expression of IL-6 in dental pulp tissue was detected by immunohistochem-istry;the expression of IL-6/STAT3 signaling pathway-related proteins was detected by western blotting.Results:Compared with Control group,rats in Model group had a reduced diet,not dare to bite food,and were depressed.Necrosis of dental pulp tissue,disordered ar-rangement of dentin cells,infiltration of inflammatory cells and increase of fibrous tissue,congestion and dilation of root pulp;the levels of serum IL-6,IL-1β,TNF-α,CXCL10 and VEGF,the average optical density of IL-6,the protein levels of IL-6 and JAK2,and the level of p-STAT3/STAT3 were significantly increased(P＜0.05).Compared with Model group,rats in SHI-L,SHI-M and SHI-H groups had a normal diet,a better mental state,and reduced pathological changes in dental pulp tissue.The levels of serum IL-6,IL-1β,TNF-α,CX-CL10 and VEGF,IL-6 average optical density,IL-6 and JAK2 protein levels,and p-STAT3/STAT3 levels gradually decreased(P＜0.05).Compared with SHI-H group,rats in SHI-H+Colivelin group had poor diet and poor spirit,pulp tissue lesions aggravated.The levels of serum IL-6,IL-1β,TNF-α,CXCL10 and VEGF,the average optical density of IL-6,the protein levels of IL-6 and JAK2,and the level of p-STAT3/STAT3 were significantly increased(P＜0.05).Conclusion:SHI can inhibit the level of inflammation in pulpitis rats and reduce the damage of pulp tissue,the mechanism may be relate to the inhibition of IL-6/STAT3 signaling pathway.
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