A study on the Correlation between Vaginal Flora Diversity and Cervical Cancer
Objective:To study the changes in the composition of vaginal flora in women with cervical cancer disease and to ex-plore vaginal probiotics with the potential to prevent the development of cervical cancer.Methods:Twenty-four healthy women and 43 women with cervical cancer were selected from the Cancer Hospital of Xinjiang Medical University and community collections.Their vaginal flora composition was analysed using 16S rDNA and macro-genome sequencing techniques.Results:There was no statistically significant difference between the vaginal flora of the two groups of women in chaol index and ASV number,suggesting a similar com-munity abundance,while in Shannon and Simpson indices,which indicate species diversity,the cervical cancer group showed a signifi-cant increase,and the difference was statistically significant.At the phylum level of microbial classification,the thick-walled phylum was the main species of vaginal microorganisms in both groups,but the proportion of the cervical cancer group(45％)decreased compared with that of the healthy group(91％),while the proportion of the rest,such as Ascomycetes and Actinomycetes,was significantly higher.At the species level,the proportion of Lactobacillus spp.in the cervical cancer group(14.2％)decreased significantly compared to that in the healthy group(81％).On the basis of macro-genome sequencing,it was concluded that Lactobacillus convolvulus has a probiotic sig-nature significance in healthy females and may exert a probiotic effect in the function of the ABC transporter,using LEfSe and LDA dis-criminant analysis.Conclusion:Women with cervical cancer are characterised by an increase in vaginal microbial diversity and a de-crease in the level of Lactobacillus,in which the different species at the level of microbial species was explored by macro-genome se-quencing technology to conclude that Lactobacillus convolvulus has a potential probiotic role in the function of the ABC transporter in the prevention of the development of cervical cancer,and this can provide theoretical evidence for the prevention of cervical carcinogene-sis from the perspective of regulating the vaginal micro-ecology for the future clinic.
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