Correlation Analysis of Peripheral Blood miR-122a and miR-146a Levels and Th1/Th2/Th17 Immune Balance in Children with Atopic Dermatitis
Objective:To investigate the correlation between peripheral blood microRNA(miRNA)-122a and miR-146a levels and T helper cells(Th)1/Th2/Th17 immune balance in children with atopic dermatitis(AD).Methods:100 children with AD who were admitted to the Dermatology Department of Shijiazhuang Maternal and Child Health Hospital from May 2020 to May 2023 were selected as AD group.According to scoring atopic dermatitis(SCORAD),they were divided into mild group with 31 cases,moderate group with 41 cases and severe group with 28 cases.Another 100 healthy children during the same period were selected as control group.Real-time fluorescence quantitative polymerase chain reaction was used to detect the levels of peripheral blood miR-122a and miR-146a,and flow cytometry was used to detect the proportion of peripheral blood Th1,Th2 and Th 17 cells.The levels of peripheral blood Th1,Th2 and Th17-related cytokines[interleukin(IL)-2,interferon-γ(IFN-γ),IL-4,IL-13,IL-17,IL-22]were detected by enzyme-linked immunosor-bent assay,and the ratio of Th1/Th2/Th17 was calculated.The correlations of peripheral blood miR-122a,miR-146a and Thl,Th2,Th 17 and related cytokines and Th1/Th2/Th17 of children with AD were analyzed by Pearson/Spearman correlation analysis.Results:The lev-els of peripheral blood miR-122a,miR-146a,Th1,Th1/Th2/Th17,IL-2 and IFN-y of the AD group were lower than those of the control group,and the levels of Th2,Th17,IL-4,IL-13,IL-17 and IL-22 were higher than those of the control group(all P＜0.05).The levels of peripheral blood miR-122a,miR-146a,Th1,Th1/Th2/Th17,IL-2 and IFN-y of the mild group,moderate group and severe group were decreased successively,while the levels of Th2,Th17,IL-4,IL-13,IL-17 and IL-22 were increased successively(all P＜0.05).Pear-son/Spearman correlation analysis showed that peripheral blood miR-122a and miR-146a in children with AD were positively correlated with the levels of Th1,Th1/Th2/Th17,IL-2,and IFN-y(r/rs were 0.679,0.677,0.684,0.706,0.693,0.689,0.671,0.694,respectively,all P＜0.001),and negatively correlated with Th2,Th17,IL-4,IL-13,IL-17,IL-22(r/rs were-0.690,-0.680,-0.681,-0.669,-0.675,-0.676,-0.676,-0.686,-0.682,-0.674,-0.680,-0.689,respectively,all P＜0.001).Conclusion:The decreased levels of peripheral blood miR-122a and miR-146a of patients with AD are closely related to the severity of the disease,and which may participate in the occur-rence and development of AD by regulating the Th1/Th2/Th17 immune balance.
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