Relationship between the Expression of LncRNA DCST1-AS1 and LncRNA MCM3AP-AS1 in Cervical Squamous Cell Carcinoma Tissue and the Proliferation and Invasion Genes and Prognosis
Objective:To investigate the relationship between the expression of long non-coding ribonucleic acids(LncRNA)DC-STAMP domain 1-antisense 1(DCST1-AS1)and LncRNA MCM3AP antisense RNA 1(MCM3AP-AS1)in cervical squamous cell carcinoma(CSCC)tissue and the proliferation and invasion genes and prognosis.Methods:124 CSCC patients admitted to The Second Affiliated Hospital of Soochow University from January 2018 to January 2020 were selected,CSCC tissues and cancer adjacent normal tissues were taken,the mRNA expression of LncRNA DCST1-AS1,LncRNA MCM3AP-AS1,proliferation genes(YAP1,Piwil2,EZH2,PKCε)and invasion genes(Rab1 1,TUG1,Gli1,FoxM1)were detected by real-time fluorescence quantitative polymerase chain reaction(qRT-PCR).Patients were followed up for 3 years after discharge.The correlation between Lnc RNA DCST1-AS1,Lnc RNA MCM3AP-AS1 expression and proliferation genes and invasion genes were analyzed by Pearson correlation analysis.Analyzed the rela-tionship between the expression of Lnc RNA DCST1-AS1,Lnc RNA MCM3AP-AS1 and clinical pathological features.The prognosis was analyzed by plotted Kaplan-Meier curve,and the factors affecting the prognosis of CSCC patients were analyzed by multivariate COX regression analysis.Results:The expression of Lnc RNA DCST1-AS1 in CSCC cancer tissues was higher than that in cancer adja-cent tissues(P＜0.05),and the expression of Lnc RNA MCM3 AP-AS1 was lower than that in cancer adjacent tissues(P＜0.05).The mR-NA levels of YAP1,Piwil2,EZH2,PKCε,Rab11,TUG1,Gli1 and FoxM1 were positively correlated with the expression of Lnc RNA DCST1-AS1 in CSCC tissues(P＜0.05),and negatively correlated with Lnc RNA MCM3AP-AS1(P＜0.05).The expression of Lnc RNA DCST1-AS1 in cancer tissues of CSCC patients with low-moderate differentiation,FIGO stage Ⅲa and pelvic lymph node metastasis was higher than that in patients with high differentiation,FIGO stage Ⅰ～Ⅱ and no pelvic lymph node metastasis(P＜0.05),the expression of Lnc RNA MCM3 AP-AS1 was lower than that in patients with high differentiation,FIGO stage Ⅰ～Ⅱ and no pelvic lymph node metasta-sis(P＜0.05).The 3-year overall survival rate of CSCC patients with high expression of Lnc RNA DCST1-AS1 and low expression of Lnc RNA MCM3AP-AS1 was lower than that of low expression of Lnc RNA DCST1-AS1 and high expression of Lnc RNA MCM3AP-AS1(P＜0.05).Multivariate COX regression analysis showed that,pelvic lymph node metastasis and high expression of Lnc RNA DC-ST1-AS1 were risk factors for poor prognosis in patients with CSCC(P＜0.05),and high expression of Lnc RNA MCM3AP-AS1 was a protective factor(P＜0.05).Conclusion:The expression of LncRNA DCST1-AS1 is up-regulate and the expression of LncRNA MCM3AP-AS1 is down-regulate in CSCC tissues,which is relate to the proliferation,invasion and poor prognosis of CSCC.
Cervical squamous cell carcinomaLncRNA DCST1-AS1LncRNA MCM3AP-AS1Proliferation and invasion genePrognosis
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