基于液相色谱串联高分辨质谱技术的小细胞肺癌血浆代谢组学研究
Plasma Untargeted Metabolomics of Small Lung Cancer Cell Based on Liquid Chromatography-High Resolution Mass Spectrometry
阿衣娜扎尔·艾合买提 1李雨婧 2邹沛辰 1王珏 3赵雪雯 3沙攀 3朱亮 1唐亚斌1
作者信息
- 1. 上海交通大学基础医学院药理学与化学生物学系 上海 200025
- 2. 上海交通大学附属胸科医院呼吸内科 上海 200030
- 3. 上海交通大学医学院 上海 200025
- 折叠
摘要
目的:对小细胞肺癌患者血浆开展质谱代谢组学研究,表征其基线代谢特征,为小细胞肺癌诊疗提供基础代谢数据及线索.方法:基于液相色谱串联四极杆飞行质谱技术,以45例小细胞肺癌患者和53例对照组(健康人群)血浆为研究对象,进行非靶向代谢组学分析及代谢表征.结果:与对照组相比,基线期小细胞肺癌患者血浆代谢发生显著改变,共发现113种差异代谢物,其中2'-deoxyguanosine 5'-monophosphate(FC=9.17)、cyclic adenosine monophosphate(FC=7.72)、inosine(FC=6.38)等 59 种代谢物水平升高,2-aminobenzoic acid(FC=0.24)、glutathione(FC=0.27)、xanthine(FC=0.29)等 54 种代谢物水平下降,差异代谢通路主要涉及色氨酸等氨基酸代谢.结论:小细胞肺癌患者基线血浆代谢轮廓与健康人群存在显著差异,相关差异代谢物及通路的挖掘有助于推动小细胞肺癌新型诊断标志物发现和代谢干预靶点的揭示,改善其治疗手段有限等现状.
Abstract
Objective:Conduct metabolomic research on the plasma of patients with small cell lung cancer to characterize their baseline metabolic characteristics and provide basic metabolic data and clues for the treatment of small cell lung cancer.Methods:Based on liquid chromatography couple with quadrupole time-of-fight mass spectrometry,plasma untargeted metabolomics studies and metabolic characterization on 53 controls(healthy people)and 48 patients with small cell lung cancer were developed.Results:The plasma metabolism was significantly altered in patients with small cell lung cancer at baseline compared to controls and a total of 113 differential metabolites were identified.The 59 up-regulated metabolites such as 2'-deoxyguanosine 5'-monophosphate(FC=9.17),cyclic adenosine monophosphate(FC=7.72),and inosine(FC=6.38),and 54 down-regulated metabolites such as 2-aminobenzoic acid(FC=0.24),glutathione(FC=0.27)and xanthine(FC=0.29),mainly participated in amino acid metabolism.Conclusions:Metabolic profiling was changed significantly between patients with small cell lung cancer and controls.The discovery of relevant differential metabolites and pathways will help promote the development of new targeted drugs for small cell lung cancer,thereby improving the current situation of limited treatment options and lack of targeted treatments.
关键词
小细胞肺癌/血浆/代谢组学/高分辨质谱Key words
Small Cell Lung Cancer/Plasma/Metabolomics/High Resolution Mass Spectrometry引用本文复制引用
出版年
2024
NETL
NSTL
万方数据