Green Synthesis of Silver Nanoparticles of Arnebia Euchroma and Their Anti-superficial Fungi Effects
Objective:Simple and rapid synthesis of silver nanoparticles using Arnebia euchroma extract as a reducing agent and silver nitrate as a silver source for characterization and anti-superficial fungi effects.Methods:The absorbance of the synthesized silver nanoparticles was used as an index to investigate the effects of the amount of comfrey extract,the concentration of AgNO3 solution,and reaction pH on the synthesis of silver nanoparticles of A.euchroma(ZC-AgNPs),and the response surface optimization was applied to screen the optimal synthesis conditions.The physicochemical properties of ZC-AgNPs were characterized by ultraviolet-visible spec-trophotometry(UV-Vis),dynamic laser scattering(DLS),transmission electron microscopy(TEM),X-ray powder diffraction(XRD),and Fourier-transform infrared spectroscopy(FTIR).Chemical silver nanoparticles(C-AgNPs)were also prepared by the chemical reduction method,and the minimum inhibitory concentration(MIC)of ZC-AgNPs and C-AgNPs against superficial fungi was tested using the mi-crodilution method.Results:The optimal process for ZC-AgNPs was a silver nitrate concentration of 10 mmol/L,a volume of comfrey extract 1.7 mL,and a pH of 10.8.The obtained ZC-AgNPs were spherical,with an average particle size of about 12.59 nm analyzed by transmission electron microscopy,and were uniformly distributed and stable.The results of the antifungal assay against superficial fungi showed that all ZC-AgNPs were superior to C-AgNPs.The MIC of ZC-AgNPs against Trichophyton rubrum,T.mentagrophyte,and Can-dida albicans were 62.5,125,and 15.63 μg/mL,respectively.The MIC of C-AgNPs against T,rubrum,T.mentagrophytes,and Candida albicans were 250,500,and 250 μg/mL,respectively.Conclusions:The preparation method of ZC-AgNPs is stable,feasible,and has a good antifungal effects against superficial fungi.
Green synthesisSilver nanoparticlesAmebia euchromaSuperficial fungi
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