Effect of Suhexiang Pill on Regulation the cAMP-PKA Signal Pathway on Neuroinflammation and Neuron Apoptosis in Rats with Acute Cerebral Infarction
Objective:To investigate the effect of Suhexiang Pill on regulating the cyclic adenosine monophosphate(cAMP)/pro-tein kinase A(PKA)signaling pathway on neuroinflammation and neuronal apoptosis in rats with acute cerebral infarction.Methods:SD rats were divided into model group,sham surgery group,low-dose Suhexiang pill group(2.5 mL/kg),medium dose Suhexiang pill group(5 mL/kg),high-dose Suhexiang pill group(10 mL/kg),nimodipine group(2 mg/kg),SQ22536(cAMP inhibitor)group(2.13 mg/kg),and high-dose Suhexiang pill+SQ22536 group(10 mL/kg+2.13 mg/kg),with 24 rats in each group.Except for the sham operated group where only the arteries were isolated,all other groups of rats were required to construct acute cerebral infarction models.After successful mod-eling,medication treatment was performed once a day for 2 weeks.Zea Longa scoring method was used to detect neural function;Dry wet weight method was used to detect the water content of rat brain tissue;Measure the volume of cerebral infarction with 2,3,5-triph-enyltetrazole chloride(TTC)staining;Enzyme-linked immunosorbent assay(ELISA)was used to detect tumor necrosis factor-α(TNF-α),Interleukin(IL)-6,IL-1β,CAMP level in rat brain tissue;Hematoxylin eosin(HE)staining was used to detect pathological changes in rat neurons;TUNEL staining was used to detect neuronal apoptosis;Western blot was used to detect the expression of cleaved aspartate spe-cific cysteine protease-3(Cleaved Caspase-3),Bcl-2 related X protein(Bax),and p-PKA protein in rat brain tissue.Results:Compared with the sham surgery group,neurological function score,neuronal apoptosis rate,TNF-α,IL-1 β,IL-6 levels,cerebral infarction volume,Bax,Cleaved Caspase-3 protein expression,and brain tissue water content increased in model group,the expression of cAMP and p-PKA proteins was reduced(P＜0.05),and the pathological damage to the CA1 region of the hippocampus in brain tissue was severe.Compared with the model group,the low-dose Suhexiang pill group,medium dose Suhexiang pill group,high-dose Suhexiang pill group,nimodip-ine group of rat neurological function score,TNF-α,IL-1 β,IL-6 level,brain tissue water content,neuronal apoptosis rate,cerebral in-farction volume,Bax,Cleaved Caspase-3 protein expression decreased,CAMP and p-PKA protein expression increased(P＜0.05),allevi-ation of pathological damage in hippocampal CA1 region of brain tissue,the trend of changes in corresponding indicators in the SQ22536 group was opposite to the above(P＜0.05);SQ22536 weakened the inhibitory effect of high-dose Suhexiang Pill on neuroinflammation and neuronal apoptosis in rats with acute cerebral infarction.Conclusion:Suhexiang Pill may inhibit neuroinflammation and neuronal apoptosis in rats with acute cerebral infarction by activating the cAMP/PKA pathway.
Suhexiang PillcAMP-PKA signal pathwayAcute cerebral infarctionNeuroinflammationNeuronal apoptosis
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