Study on the Relationship between the Expression of microRNA-93-5p and microRNA-98-5p in Papillary Thyroid Carcinoma and Clinicopathological Features,Proliferation and Invasion Gene Expression
Objective:To study the relationship between the expression of micro-93-5p(miR-93-5p)and microRNA-98-5p(miR-98-5p)in papillary thyroid carcinoma(PTC)and clinicopathological features,proliferation and invasion gene expression.Methods:153 PTC patients who were underwent surgical resection in Guangdong Provincial Hospital of Traditional Chinese Medicine from Octo-ber 2020 to October 2023 were selected as the research objects,and the intraoperatively resected cancer tissues and adjacent tissues were collected.The expression levels of miR-93-5p,miR-98-5p,proliferation gene and invasion gene mRNA in cancer tissues and adjacent tis-sues were detected and compared,and the relationship between the expression of miR-93-5p and miR-98-5p and the clinicopathological features of PTC patients were analyzed.The correlation between the expression levels of miR-93-5p and miR-98-5p and the mRNA expression of proliferation genes and invasion genes were analyzed by Pearson method.Results:The expression level of miR-93-5p in cancer tissues was higher than that in adjacent tissues,and the expression level of miR-98-5p was lower than that in adjacent tissues(P＜0.05).The proportion of TNM stage Ⅲ～Ⅳ,lymph node metastasis and poor differentiation in PTC patients with high expression of miR-93-5p were higher than those in PTC patients with low expression of miR-93-5p(P＜0.05).The proportion of TNM stage Ⅲ-Ⅳ,lymph node metastasis and poor differentiation in PTC patients with low expression of miR-98-5p were higher than those in PTC patients with high expression of miR-98-5p(P＜0.05).The levels of proliferation gene programmed cell death factor 4(PDCD4)and protein phos-phatase 4 regulatory subunit(PP4R1)in cancer tissues were lower than those in adjacent tissues(P＜0.05),and the levels of invasion gene metalloprotease disintegrin 9(ADAM9)and Bcl-6 co-inhibitor-like protein(BCORL1)were higher than those in adjacent tissues(P＜0.05).Pearson analysis showed that,the expression of miR-93-5p was negatively correlated with the expression levels of proliferation genes PDCD4 and PP4R1,and positively correlated with the expression levels of invasion genes ADAM9 and BCORL1.The expression level of miR-98-5p was positively correlated with the levels of proliferation genes PDCD4 and PP4R1,and negatively correlated with the expression levels of invasion genes ADAM9 and BCORL1.Conclusion:The expression of miR-93-5p in cancer tissues of PTC patients is increase,and the expression of miR-98-5p is decrease,which are relate to clinicopathological features such as TNM stage,lymph node metastasis and differentiation degree,and can also promote the proliferation and invasion of PTC cancer cells.
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