Analysis of Intestinal Flora Characteristics in Preschool Children with Obstructive Sleep Apnea Hypopnea Syndrome
Objective:To investigate the difference of intestinal flora between preschool children with obstructive sleep apnea hy-popnea syndrome(OSAHS)and normal children.Methods:30 preschool children with OSAHS admitted to the pediatric outpatient de-partment of the First Affiliated Hospital of Xinjiang Medical University from July 2023 to November 2023 were selected as OSAHS group,and 30 healthy children who underwent physical examination in the health management center of our hospital during the same period were selected as control group.The intestinal flora was analyzed by 16 SrDNA amplicon sequencing technology.The correlation between sleep quality and the abundance of intestinal flora at phylum and genus levels were analyzed by Spearman method.Results:2588 amplicon sequence variations(ASVs)were found in OSAHS group and control group,1034 unique ASVs were detected in OSAHS group and 1554 unique ASVs were detected in control group.Chao1 index and Observed otus index in OSAHS group were significantly lower than those in control group(P＜0.05),there was no significant difference in shannnon index,Simpson index,Goods coverage index and peilou-e index between two groups(P＞0.05).There was a significant difference in the structure of intestinal flora between OSAHS group and control group(P＜0.05).At the phylum level,the relative abundance of Bacteroidetes in OSAHS group was lower than that in control group,and the relative abundance of Firmicutes and the ratio of Firmicutes/Bacteroidetes were higher than those in control group(P＜0.05).At the genus level,there was no significant difference in the relative index of dominant bacteria between OSAHS group and control group(P＞0.05).At the phylum level,sleep quality was positively correlated with Bacteroidetes(P＜0.05).At the genus level,sleep quality was negatively correlated with Bifidobacterium and Lactobacillus,and positively correlated with Bacteroides(P＜0.05).Obstruc-tive apnea hypopnea index(OAHI)was negatively correlated with Enterobacter(P＜0.05).The lowest oxygen saturation(LSaO2)was pos-itively correlated with Enterobacter(P＜0.05).Mean oxygen saturation(MSaO2)was positively correlated with X.Eubacterium._eli-gens_group(P＜0.05).Conclusion:There are differences in the types and relative abundance of intestinal flora between preschool OSAHS children and normal children,the proportion of Firmicutes/Bacteroidetes is increase,and there may be intestinal flora imbalance.Sleep quality is significantly correlate with Bacteroidetes,Bifidobacterium,and Lactobacillus at the phylum and genus levels.
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