Study on the Relationship between the Expression of LncRNA MALAT1 and miR-145 and the Severity of Retinopathy of Prematurity
Objective:To investigate the relationship between the expression of long non-coding ribonucleic acids(RNA)lung adenocarcinoma metastasis-associated transcript 1(LncRNA MALAT1)and microRNA-145(miR-145)and the severity of retinopathy of prematurity(ROP).Methods:82 premature infants with ROP admitted to our hospital from January 2021 to December 2023 were selected,according to the severity of the condition,there are 38 cases in the severe group and 44 cases in the mild group.Another 40 healthy pre-mature infants in our hospital during the same period were selected as control group.The expressions of serum LncRNA MALAT1 and miR-145 were compared in three groups,the relationship between the expressions of serum LncRNA MALAT1 and miR-145 and the severity of ROP was analyzed by Pearson correlation.The influencing factors of ROP in premature infants were analyzed by univariate and multivariate Logistic regression models.The predictive value of serum LncRNA MALAT1 and miR-145 for ROP in premature in-fants was analyzed by receiver operating characteristic(ROC)curve.Results:The expression of serum LncRNA MALAT1 in severe group was significantly higher than that in mild group and control group,and the expression of miR-145 was significantly lower than that in mild group and control group.The expression of LncRNA MALAT1 in mild group was significantly higher than that in control group,and the expression of miR-145 was significantly lower than that in control group(P＜0.05).Serum miR-145 expression was negatively correlated with the severity of ROP(r=-0.412,P＜0.05),and LncRNA MALAT1 expression was positively correlated with the severity of ROP(r=0.434,P＜0.05).Three groups of gestational age,birth weight＜1500 g constituent ratio,birth 1min Apgar score＜7 points,birth 5min Apgar score＜7 points,the incidence of maternal gestational diabetes was statistically significant(P＜0.05).Elevated LncRNA MALAT1,decreased miR-145,gestational age≤ 31 weeks,birth weight＜1500 g,and maternal gestational diabetes were risk factors for ROP in preterm infants(P＜0.05).Serum LncRNA MALAT1 and miR-145 had high sensitivity and specificity in predicting the severity of ROP,the area under the curve(AUC)of combined detection of ROP severity was greater than that of the above indicators alone.Conclu-sion:The increase of serum LncRNA MALAT1 expression and the decrease of miR-145 expression in ROP premature infants are relate to the aggravation of ROP severity,the detection of serum LncRNA MALAT1 combine with miR-145 has certain value in predicting the severity of ROP.
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