miR-27a Targets FOXG1 to Regulate Proliferation,Invasion and Migration of Cutaneous Squamous Cell Carcinoma Cells
Objective:To investigate the effects of miR-27a on Proliferation,invasion and migration of cutaneous squamous cell carcinoma(CSCC)cells and its targeting relationship with forkhead box G1(FOXG1).Methods:30 cases CSCC tissues and 30 cases normal skin tissues were collected.A431 cells were cultured.miR-NC,miR-27a mimics,si-NC,si-miR-27a,Scramble,si-FOXG1,Vec-tor,and OE-FOXG1 plasmids were transfected into the cells and defined as miR-NC group,miR-27a group,si-NC group,si-miR-27a group,Scramble group,si-FOXG1 group,Vector group and FOXG1 group.The si-FOXG1 and Scramble plasmids were transfected into the cells of miR-27a group and recorded as miR-27a+Scramble group and miR-27a+si-FOXG1 group.The cell Proliferation ability was detected by tetramethylazole blue(MTT).The cell invasion and migration ability were detected by Transwell assays.FOXG1 gene mRNA and miR-27a level were detected by fluorescence quantitative polymerase chain reaction(RT-PCR).FOXG1 protein level were detected by Western blot.TargetScan online website was used to predict the binding site of miR-27a and FOXG1.Dual luciferase reporter gene assay was used to verify the targeting relationship between miR-27a and FOXG1.Results:The levels of FOXG1 gene mRNA and miR-27a in CSCC tissues were higher than normal skin tissues(P＜0.05),and the levels of FOXG 1 gene mRNA in CSCC tissues were positively correlated with and miR-27a level(r=0.801,P=0.000).Up-regulation of miR-27a and FOXG1 promoted cell prolifera-tion,invasion and migration,and silencing of miR-27a and FOXG1 inhibited cell proliferation,invasion,migration and EMT.Down-reg-ulation of miR-27a reversed the the promotive effect of cell Proliferation,invasion and migration by overexpression of FOXG 1.miR-27a positively regulated FOXG1 expression.Conclusion:miR-27a and FOXG1 are highly expressed in CSCC tissues.miR-27a positively regulates FOXG1 to promote proliferation,invasion,and migration of CSCC cells.
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