Effects of NLRP3 Inflammation Inhibitor BAY-117082 on Epithelial-Mesenchymal Transition in HSC-2 Cell Line
Objective:To evaluate the effect of selective NLRP3 inflammasome inhibitor BAY-117082 on the growth and epithe-lial mesenchymal transition process of oral squamous cell carcinoma.Methods:An OSCC model was constructed on the tongue of nude mice using HSC-2 cell lines.The mice were randomly divided into group A,B,C,and D.Group A was the blank control group,while groups B,C,and D were the experimental group.Group C and D were intraperitoneally injected with BAY-117082 at doses of 2.5 mg/kg and 5 mg/kg,respectively.The tongue tumors,lymph nodes,and lung metastases were analyzed by immunohistochemistry,PCR,and Western blot.Results:An appropriate dose of BAY-117082 can significantly inhibit the growth of oral squamous cell carcinoma HSC-2 cells and the expression of N-cadherin,an intermediate cell marker,while promoting the expression of E-cadherin,an epithelial cell marker.The effect becomes more pronounced with the increase of BAY-117082 concentration.Conclusions:The appropriate concentra-tion of BAY-117082 can effectively inhibit the epithelial mesenchymal transition process of HSC-2 cell lines and effectively inhibit tumor growth,which can be used as a promising treatment strategy for OSCC.
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