MiR-194-5p Targeted Regulation of HMGA2 to Inhibit EMT Induced by TGF-β1 in Pancreatic Cancer
Objective:To investigate the targeted regulation of high mobility group A2(HMGA2)expression by miR-194-5p,thereby inhibiting epithelial mesenchymal transformation(EMT)induced by transforming growth factor(TGF)-β 1 in pancreatic cancer cells.Methods:Human pancreatic cancer cell line BxPc-3 was divided into four groups:control group,treatment group,Sh-miR-194-5p group and Sh-NC group.Except the control group,the other three groups were treated with TGF-β1 in vitro to induce and cultivate for 72 h,what's more,miR-194-5p silencing plasmid(Sh-miR-194-5p)and control plasmid(Sh-NC)were constructed to transfect cells 2 h before TGF-β1 induced culture.The morphological changes of cells were observed under inverted microscope,qRT-PCR was to detect miR-194-5p,Western blot was to detect protein expression of HMGA2 and EMT markers(E-cadherin,Vimentin,and N-cadherin),Tran-swell assay was to detect the invasiveness of cells.Results:Compared with control group,the cell morphology of the treatment group changed to a long spindle shape,exhibiting characteristic morphological changes of EMT;the expression levels of miR-194-5p,HMGA2,Vimentin,and N-cadherin were significantly higher,while the expression level of E-cadherin was significantly lower,the number of inva-sive cells was less,too(P＜0.05).Compared with the treatment group and Sh-NC group,the morphological changes of cells in the Sh-miR-194-5p group were significantly less,but still more than the control group,the expression levels of miR-194-5p,HMGA2,Vi-mentin,and N-cadherin were significantly lower,but the expression level of E-cadherin was significantly more and number of invasive cells was more,too(P＜0.05),the differences between the Sh-miR-194-5p group and the control group were still statistically significant(P＜0.05).Conclusion:Human pancreatic cancer cells may overexpress miR-194-5p,which partially inhibits cell EMT changes induced by TGF-β1 via targetly regulating HMGA2 expression.It can provide novel targets for disease pathogenesis and clinical intervention.
Pancreatic cancerEpithelial mesenchymal transitionTransforming growth factor-β1High mobility group A2MiR-194-5p
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