Value Analysis of LAMP Technology in Clinical Diagnosis of Pulmonary Tuberculosis
Objective:The aim of this study is to investigate the potential application of loop-mediated isothermal amplification technology(LAMP)in the diagnosis of pulmonary tuberculosis.Methods:The clinical data of 305 patients with suspected tuberculosis admitted to Baoding Second Central Hospital from June 2020 to June 2023 were retrospectively analyzed.Based on the pathological re-sults,the non-tuberculosis group(123 cases)and tuberculosis group(182 cases)were included.The sputum samples with different traits,using modified Roche culture method,smear acid stain method and LAMP detection of Mycobacterium tuberculosis.Results:Compared with the smear acid-fast staining method and the improved Roche culture method,the positive rate of LAMP samples was relatively high,and the positive rate of LAMP samples with cheese sputum was relatively high compared with other samples.The results of acid-fast staining showed that 80 cases were positive,119 cases were negative,4 cases were misdiagnosed and 102 cases were missed.The results of modified Roche culture showed that 104 cases were positive,120 cases were negative,3 cases were misdiagnosed and 78 cases were missed.LAMP results showed that 135 cases were positive,121 cases were negative,2 cases were misdiagnosed and 47 cases were missed.Compared with smear acid staining method and modified Roche culture method,the sensitivity,negative predictive value,accu-racy and area under the curve(AUC)value of LAMP examination were relatively high,in which the sensitivity,accuracy and AUC value of smear acid staining method were lower than those of modified Roche culture method(P＜0.05).Conclusion:LAMP technique was ben-eficial to the identification of Mycobacterium tuberculosis in various trait samples,and had high diagnostic sensitivity,negative predictive value and accuracy for Mycobacterium tuberculosis in sputum samples,which had higher diagnostic value for pulmonary tuberculosis.
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