Protective Effect of Smilagenin on MPP+-induced Apoptosis in SH-SY5Y Cells
Objective:The purpose of this article was to investigate the exerting protective effect of smilagenin on MPP+-induced apoptosis in SH-SY5Y cells.Methods:In the experiment,PD cell model was constructed using MPP+treated human neuroblastoma SH-SY5Y cells.SH-SY5Y cells in logarithmic growth phase were taken and divided into four groups:the Control group(Control),the MPP+group(model group),the smilagenin group(10 μM),and the MPP++smilagenin group(3 mm,10 µM).The cell proliferation as-say(CCK-8)was firstly used to detect the optimal concentration of smilagenin action and the effect on SH-SY5Y cell viability.The effect of smilagenin on the apoptosis rate of SH-SY5Y cells was detected by AnnexinV-FITC/PI double staining assay,and the protein expres-sion of apoptosis-related proteins Bax,Bcl-2 was detected by protein immunoblotting assay.Results:The cell viability assay determined 10 μM/mL of smilagenin as the added concentration for the subsequent experiments.Compared with the control group,the MPP+group showed significantly lower cell viability(P＜0.001),higher apoptosis rate(P＜0.001),and significantly higher expression of apoptosis-re-lated protein Bax/Bcl-2(P＜0.01).Compared with the MPP+group,cell viability was significantly higher in the MPP++smilagenin group(P＜0.001),apoptosis rate was significantly lower(P＜0.01),apoptosis-related protein Bax/Bcl-2 expression was significantly lower(P＜0.01).Conclusions:Smilagenin can inhibit MPP+-induced apoptosis in SH-SY5Y cells,which may be related to its regulation of pro-tein expression of apoptosis-related proteins Bax and Bcl-2.
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