eIF3A and eIF4E Synergistically Regulate Proliferation and Apoptosis of Cervical Cancer Cells
Objective:To investigate the synergistic regulation of cervical cancer cell proliferation and apoptosis by eIF3A and eIF4E.Methods:Firstly,human cervical cancer cell lines HeLa,SiHa and normal cervical epithelial cell HcerEpic with logarithmic growth phase were selected.Real-time fluorescence quantitative PCR(qRT-PCR)and Western blot were used to detect eIF 3A,eIF4E mRNA and protein expression levels,respectively.Then lentiviral vectors for silent expression of eIF 3A and eIF4E were constructed and transfected SiHa cell,stable expression strains were screened using qRT-PCR and Western blot.Finally,SiHa cells were divided into four groups,namely the control group,eIF 3A low-expression group,eIF 4E low-expression group,and eIF 3A+eIF4E low-expression group.After continuous cultivation for 48 hours,cell proliferation rate was measured using CCK8 assay,cell apoptosis rate was measured using flow cytometry,and the expression levels of Ki-67 and cleared-Caspase-3 proteins were detected using Western blot.Results:① Com-pared with normal cervical cells,the expression levels of eIF 3A,eIF4E mRNA and protein in HeLa and SiHa were significantly higher(P＜0.05).② Compared with the control group,the eIF 3A low-expression group significantly reduced the expression of eIF 3A mRNA and protein;the expression levels of eIF 4E mRNA and protein were significantly less in the eIF 4E low-expression group;the expression levels of eIF 3A,eIF4E mRNA and protein were both significantly lower in the eIF 3A+eIF4E low-expression group(P＜0.05).③Com-pared with the control group,the eIF 3A low-expression group,eIF 4E low-expression group and the eIF 3A+eIF4E low-expression group showed decrease of cell proliferation rate,increase of cell apoptosis rate,less of Ki-67 protein expression,and higher of cleared-Casase-3 protein expression(P＜0.05).Conclusion:eIF 3A and eIF4E may synergistically exert biological functions in regulating cell proliferation and apoptosis of cervical cancer.
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