Cryptotanshinone Protects Human Bone Marrow Mesenchymal Stem Cells from Hydrogen Peroxide Induced Cell Apoptosis by Regulating the SDF-1/CXCR4 Signaling Pathway
Objective:To investigate the mechanism by cryptotanshinone regulates the signaling pathway of stromal cell derived factor 1(SDF-1)/C-X-C chemokine receptor 4(CXCR4)and inhibits hydrogen peroxide induced cell apoptosis of human bone marrow mesenchymal stem cells(BMSCs).Methods:Cultivated human bone marrow mesenchymal stem cells(HBMSCs)in vitro and established an oxidative stress model by H2O2.Divided HBMSC cells into control group(without oxidative stress induction)and model group(500 μmol/L H2O2 induction for 24 hours),low-dose group of cryptotanshinone(10 μmol/L),medium dose group of cryptotanshinone(20 p.mol/L),high-dose group of cryptotanshinone(40 pmol/L).Cell activity was detected by Cell Count Kit(CCK-8).Cell apoptosis was detected by flow cytometry detection.Cellular glucose uptake and lactate levels was detected by enzyme linked immunosorbent assay(Elisa)testing.The content of glutathione(GSH),superoxide dismutase(SOD)and malondialdehyde(MDA)in cells were detected by micro method.Cysteine aspartate protease 3(caspase-3),B-lymphoblastoma-2 associated X protein(Bax),B-lymphoblastoma-2(Bcl-2),expression of SDF-1 and CXCR4 proteins was detected by western blot detection(Western blot).Results:Compared with the control group,the glucose uptake and lactate levels,GSH,SOD activity,Bcl-2 protein expression,and SDF-1/CXCR4 signaling pathway protein expression of HBMSC cells in the model group decreased,while the cell apoptosis rate,expression of apoptotic protein caspase-3,Bax,and MDA content increased(P＜0.05).Compared with the model group,the low,medium,and high dose groups of cryptotanshinone showed an increase in glucose uptake and lactate levels,GSH,SOD activity,Bcl-2 protein expression,and SDF-1/CXCR4 signaling path-way protein expression in HBMSC cells,while the cell apoptosis rate,expression of apoptotic proteins caspase-3,Bax,and MDA content decreased(P＜0.05).The differences in various indicators among the low,medium,and high dose groups of cryptotanshinone were statis-tically significant(P＜0.05).Conclusion:Cryptotanshinone may inhibit oxidative stress damage and apoptosis of BMSC induced by hydro-gen peroxide by promoting SDF-1/CXCR4 signaling pathway transduction,with high-dose cryptotanshinone having the best effect.
CryptotanshinoneHuman bone marrow mesenchymal stem cellsSDF-1/CXCR4 signaling pathwayOxidative stressCell apoptosis
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