Relationship between NLRP 3 Inflammasome and Disease Activity and Bone Metabolism Indexes of Rheumatoid Arthritis Patients and its Diagnostic Value Analysis
Objective:To study the relationship between nucleotide-binding oligomerization domain-like receptors family pyrin domain containing 3(NLRP3)inflammasome and disease activity and bone metabolism indexes of rheumatoid arthritis(RA)patients and its diagnostic value.Methods:300 RA patients admitted to the First Hospital of Shanxi Medical University from May 2021 to September 2022 were selected,and the patients were divided into low-degree group(n=62),moderate group(n=104),severe group(n=134)according to the 28 joint disease range of motion(DAS28)score.NLRP3,apoptosis-related spot-like protein(ASC),caspase-1(caspase-1)mRNA expression level,bone metabolism indexes of each group were detected and compared.The correlation of NLRP3 inflammasome index and DAS 28 score and bone metabolism indexes was analyed by Pearson correlation,and the diagnostic value of NLRP3,ASC,and cas-pase-1 for the severity of disease activity of RA was analyzed by receiver operating characteristic(ROC)curve.Results:The serum NLRP 3,ASC and Caspase-1 mRNA were higher in the severe and moderate groups than the low-degree group,and severe group was higher than the moderate group(P＜0.05).Serum Bone gla protein(BGP),parathyroid hormone(PTH)and β-collagen special sequence(β-CTx)levels in the moderate group and low-degree group were significantly lower than the severe group,and low-degree group was lower than the moderate group(P＜0.05).Serum 25-(OH)Vitamin D3[25-(OH)D3]and type Ⅰ procollagen propeptide(PINP)levels were signifi-cantly higher in the moderate group and low-degree grade group than the severe group,and low-degree grade group was higher than the moderate group(P＜0.05).The Pearson correlation analysis showed that,the serum NLRP 3 inflammasome index was positively associat-ed with DAS 28 score,BGP,PTH and β-CTx,but negatively associated with 25-(OH)D3 and PINP(all P＜0.05).ROC analysis showed that the area under the curve of NLRP3,NLRP3,ASC alone and in combination for the severity of disease activity of RAwas 0.770,0.733,0.739,and 0.828,respectively.Conclusion:The NLRP3 inflammasome is closely related to disease activity in RA patients,the serum NLRP 3 inflammasome index is positively correlated with DAS 28 score,BGP,PTH and β-CTx,but negatively correlated with 25-(OH)D3 and PINP.The combined detection of NLRP 3,ASC and Caspase-1 levels has the higher diagnostic value for the severity of disease activity of RA.
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