FYSBF Inhibited the Malignant Biological Behavior of Acute Myeloid Leukemia Cells by Regulating JAK2/STAT3 Pathway
Objective:To explore the relationship between the therapeutic effect of Fu Yuan Sheng Bai Fang(FYSBF)on acute myeloid leukemia(AML)and the JAK/STAT signaling pathway and its mechanism.Methods:AML HL-60 cells were divided into control group,FYSBF group,IL-6 group(IL-6 is the activator of JAK/STAT signaling pathway)and FYSBF+IL-6 group.HL-60 cells in control group were cultured in normal RPMI 1640 medium,HL-60 cells in FYSBF group were cultured in medium which was added with a final concentration of 200 μg/mL FYSBF,and HL-60 cells in IL-6 group were cultured in medium which was added with a final concentration of 100 ng/mL IL-6.HL-60 cells in FYSBF+IL-6 group were cultured in medium which were added with final concentration of 200 μg/mL FYSBF and 100 ng/mL IL-6.The cell proliferation was detected by CCK-8 assay and clonal formation assay,cell cycle distribution and apoptosis were detected by flow cytometry,cell invasion and migration were detected by Transwell assay,and protein expression levels were analyzed by Western blot assay.Results:Compared with the control group,the relative vitality,number of clones,number of migration and invasion cells and proportion of S-phase cells in FYSBF group were decreased(P＜0.05),and the protein expressions of CDK2,cyclin E,N-cadherin and Vimentin were decreased(P＜0.05),the phosphorylation levels of JAK2 and STAT3 proteins were decreased(P＜0.05),the cells proportion of G0/G1 phase and apoptosis rate were increased(P＜0.05),and the protein expression of p21 and E-cadherin were increased(P＜0.05).Compared with the control group,the relative vitality,number of clones,number of migration and invasion cells and proportion of S-phase cells in IL-6 group were increased(P＜0.05),and the protein expressions of CDK2,cyclin E,N-cadherin and Vimentin were increased(P＜0.05),the phosphorylation levels of JAK2 and STAT3 proteins were increased(P＜0.05),the cells proportion of G0/G1 phase and apoptosis rate were decreased(P＜0.05),and the protein expression of p21 and E-cadherin were decreased(P＜0.05).Compared with the IL-6 group,the relative vitality,number of clones,number of migration and invasion cells and proportion of S-phase cells in FYSBF+IL-6 group were decreased(P＜0.05),and the protein expressions of CDK2,cyclin E,N-cadherin and Vimentin were decreased(P＜0.05),the phosphorylation levels of JAK2 and STAT3 proteins were decreased(P＜0.05),the cells proportion of G0/G1 phase and apoptosis rate were increased(P＜0.05),and the protein expression of p21 and E-cadherin were increased(P＜0.05).Conclusion:FYSBF can inhibit the proliferation,migration,invasion and epithelial-mesenchymal transformation of acute myeloid leukemia cells,and promote cell apoptosis and cell cycle arrest,which may be related to inhibiting the activation of JAK2/STAT3 pathway.
Fu Yuan Sheng Bai Fang(FYSBF)JAK2/STAT3Acute myeloid leukemia(AML)ProliferationInvasion
NETL
NSTL
万方数据
