Study on the Relationship between the Expression of miR-146a-5p,miR-146b-5p and Angiogenesis Factors and Microvessel Density in Villus Tissue of Patients with Recurrent Spontaneous Abortion
Objective:To investigate the relationship between the expression of microRNA(miR)-146a-5p and miR-146b-5p in villus tissue of patients with unexplained recurrent spontaneous abortion(URSA)and angiogenesis factors and microvessel density(MVD).Methods:119 URSA patients who admitted to our hospital from January 2020 to December 2023 were selected as URSA group,and 90 women with normal early pregnancy who voluntarily requested abortion to terminate pregnancy were selected as control group.The expressions of miR-146a-5p,miR-146b-5p,angiogenesis factors[hypoxia-inducible factor-1α(HIF-1α),vascular endothelial growth factor(VEGF)]and MVD in villus tissues were compared between two groups.The correlation between miR-146a-5p,miR-146b-5p expression and HIF-1α,VEGF expression and MVD in villus tissue were analyzed by Pearson test.Results:Compared with control group,the expression level of miR-146a-5p was decreased in villus tissue of URSA group(P＜0.05),and the expression level of miR-146b-5p was increased(P＜0.05).Compared with control group,the MVD of villi in URSA group was decreased(P＜0.05).The expression levels of HIF-1α and VEGF in villus tissues of URSA group were lower than those in control group(P＜0.05).Pearson test showed that,the expression of miR-146a-5p in villus tissue of URSA patients was positively correlated with HIF-1α,VEGF and MVD(P＜0.05),and the expression of miR-146b-5p was negatively correlated with HIF-1α,VEGF and MVD(P＜0.05).Conclusion:The low expression of miR-146a-5p and the high expression of miR-146b-5p in villous tissues of URSA patients are related to the down-regulation of angiogenesis factors HIF-1α and VEGF and the decrease of MVD.
Unexplained recurrent spontaneous abortionVillous tissuemiR-146a-5pmiR-146b-5pAngiogenesis factorsMicrovessel density
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