Study on the Relationship between the Expression Levels of miR-139-5p and miR-379-5p of Endometrial Cancer Tissue and Invasion Related Genes and Prognosis
Objective:To study the relationship between the expression levels of microribonucleic acid(miR)-139-5p and miR-379-5p of endometrial cancer tissue and invasion related genes and prognosis.Methods:105 patients with endometrial cancer who underwent surgical treatment in The Second Affiliated Hospital of Xi'an Jiaotong University from December 2018 to December 2019 were selected.The postoperative cancer tissue and cancer adjacent tissue samples from patients were collected,and the expression levels of miR-139-5p,miR-379-5p,and invasion genes[high mobility group box 1(HMGB1),secretory protein Dikkopf-1(DKK1)]of cancer tissue and cancer adjacent tissue were detected by real time fluorescence quantitative polymerase chain reaction(PCR).Pearson method was used to analyze the correlation between the expression levels of miR-139-5p,miR-379-5p and invasion gene expression in cancer tis-sue.According to the different expression levels of miR-139-5p and miR-379-5p in cancer tissues,patients were divided into miR-139-5p high expression group(n=62)and low expression group(n=43),miR-379-5p high expression group(n=69)and low expression group(n=36),and the relationship between the high expression and low expression of miR-139-5p,and miR-379-5p and the clinical pathologi-cal characteristics of patients were analyzed.Followed up for 3 years,and patients death situation were counted.The relationship between the expression levels of miR-139-5p,miR-379-5p and the prognosis of patients with endometrial cancer were analyzed by the Ka-plan-Meier method.Results:The expression levels of miR-139-5p and miR-379-5p of cancer tissue were lower than those of cancer adja-cent tissue(P＜0.05).The expression level of DKK1 of cancer tissues was lower than that of cancer adjacent tissue,while the expression level of HMGB1 was higher than that of cancer adjacent tissue(P＜0.05).The expression levels of miR-139-5p and miR-379-5p were pos-itively correlated with DKK1 expression,and negatively correlated with HMBG1 expression(P＜0.05).The high and low expression groups of miR-139-5p and miR-379-5p showed statistically significant differences in International Federation of Gynecology and Obstet-rics(FIGO)staging,lymph node metastasis,vascular invasion,and degree of muscle infiltration(P＜0.05).105 patients were followed up for 3 years,there were 3 cases of lost visits and 21 deaths during the period,and the 3-year survival rate was 79.41％(81/102).The 3-year overall survival rate of the miR-139-5p low expression group was 61.90％(26/42),which lower than 91.67％(55/60)of the miR-139-5p high expression group.The 3-year overall survival rate of the miR-379-5p low expression group was 62.86％(22/35),which lower than 88.06％(59/67)of the miR-379-5p high expression group.Conclusion:The expression levels of miR-139-5p and miR-379-5p of endome-trial cancer tissues are downregulated,which may be related to abnormal expression of invasion genes and poor prognosis of patients.
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