Effect of P2RX3 Gene on Neurological Function Recovery in Rats with Traumatic Brain Injury
Objective:To reveal the effect of purine 2X3(P2X3)receptor(P2RX3)gene on neurological function recovery in rats with traumatic brain injury(TBI).Methods:The TBI model of rats was made by craniocerebral injury instrument.After the model establishment,54 TBI model rats were randomly divided into TBI group(n=10),TBI+NC-sh group(n=11),TBI+P2RX3-sh group(n=11),TBI+NC-OE group(n=11)and TBI+P2RX3-OE group(n=11).Rats which only cut the skin without hitting were taken as Sham group(n=10).Rats in TBI+NC-sh group,TBI+P2RX3-sh group,TBI+NC-OE group and TBI+P2RX3-OE group were injected into the brain with NC-sh,P2RX3-sh,NC-OE and P2RX3-OE respectively.Normal saline was injected into the brain of rats in Sham group and TBI group.Rats were injected again 7 days after the first injection,and the samples were taken 14 days later.The modified neurological deficit score was used to evaluate the neurological function.Cognitive function was evaluated by Morris water maze test.Sucrose preference test and open field test were used to evaluate behavior.The water content of brain tissue was measured by weighing method.Apoptosis was detected by TUNEL staining.The fluorescence intensity of BDNF was detected by immunofluorescence staining.The level of P2RX3 mRNA was detected by RT-qPCR.The protein levels of P2RX3,BDNF,TrkB,p-TrkB,protein kinase B(Akt),p-Akt,Bcl-2 and Bax were detected by Western blot.Results:Compared with TBI group and TBI+NC-sh group,the relative levels of mRNA and protein of P2RX3 in TBI+P2RX3-sh group decreased(P＜0.05),neurological function score,escape latency and brain water content were all increased(P＜0.05),platform crossing times within 60 s,sucrose preference rate and horizontal and vertical activity scores all decreased(P＜0.05),TUNEL positive rate and the relative protein level of Bax increased(P＜0.05),the relative fluorescence intensity of BDNF and the relative protein levels of Bcl-2,BDNF,p-TrkB and p-Akt decreased(P＜0.05).Compared with TBI group and TBI+NC-OE group,the relative levels of mRNA and protein of P2RX3 in TBI+P2RX3-sh group increased(P＜0.05),neurological function score,escape latency and brain water content were all decreased(P＜0.05),platform crossing times within 60 s,sucrose preference rate and horizontal and vertical activity scores all increased(P＜0.05),TUNEL positive rate and the relative protein level of Bax decreased(P＜0.05),the relative fluorescence intensity of BDNF and the relative protein levels of Bcl-2,BDNF,p-TrkB and p-Akt increased(P＜0.05).Conclusion:P2RX3 is up-regulated after TBI in rats,and up-regulation of P2RX3 can promote the recovery of neurological function,and its mechanism is related to the activation of BDNF/TrkB/Akt signal pathway.
Traumatic brain injuryPurine 2X3(P2RX3)Nerve function recoveryBDNF/TrkB/Akt signal pathway
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