卵巢癌组织lncRNA HCG18、miR-152-3p表达与临床病理特征及预后的关系研究
Study on the Relationship between the Expression of lncRNA HCG18 and miR-152-3p in Ovarian Cancer Tissues and Clinicopathological Features and Prognosis
蒋莎 1陈畅乾 1卢娟 1陈惠惠 1康旻1
作者信息
- 1. 珠海市中西医结合医院(南方医科大学附属珠海医院)妇科 广东珠海 519000
- 折叠
摘要
目的:探讨卵巢癌组织中长链非编码核糖核酸(RNA)人类白细胞抗原复合体18(lncRNAHCG18)、微小RNA-152-3p(miR-152-3p)的表达情况与患者临床病理特征及预后的关系.方法:选择2016年1月至2021年1月我院收治的卵巢癌患者185例为研究对象.采用聚合酶链式反应(PCR)法检测并比较术中获取的卵巢癌组织与癌旁组织(距离癌组织边缘＞2cm)lncRNA HCG18、miR-152-3p表达.分析lncRNAHCG18、miR-152-3p表达与卵巢癌患者临床病理特征之间的关系.对卵巢癌患者实施3年随访,采用Kaplan-Meier生存曲线比较lncRNA HCG18、miR-152-3p高表达、低表达的卵巢癌患者3年累积生存率.采用Cox风险模型分析卵巢癌患者预后的影响因素.结果:lncRNA HCG18表达水平比较,卵巢癌组织高于癌旁组织(P＜0.05),miR-152-3p表达水平比较,卵巢癌组织低于癌旁组织(P＜0.05).卵巢癌组织中lncRNA HCG18、miR-152-3p表达水平与国际妇产科联盟(FIGO)分期、分化程度、腹腔积液、淋巴结转移有关(P＜0.05).高lncRNA HCG18组3年累积生存率为41.57％(37/89),低于低lncRNA HCG18组的70.65％(65/92),差异有统计学意义(P＜0.05);高miR-152-3p组3年累积生存率75.53％(71/94),高于低miR-152-3p组的35.63％(31/87),差异有统计学意义(P＜0.05).Cox比例风险模型分析结果显示,FIGO分期Ⅲ～Ⅳ期、分化程度为高、中分化、腹腔积液、淋巴结转移、lncRNAHCG18升高,miR-152-3p降低是卵巢癌患者预后不良的危险因素.结论:卵巢癌组织中lncRNAHCG18高表达,miR-152-3p低表达与恶性临床病理特征及预后不良的发生有关.
Abstract
Objective:To investigate the expression of long non-coding ribonucleic acids(lncRNA)human leukocyte antigen complex group 18(lncRNA HCG18)and microRNA-152-3p(miR-152-3p)in ovarian cancer tissues and their relationship with clinicopathological features and prognosis of patients.Methods:185 ovarian cancer patients admitted to our hospital from January 2016 to January 2021 were selected as the study subjects.The expression of lncRNA HCG18 and miR-152-3p in ovarian cancer tissues and adjacent tissues(≥ 2 cm from the edge of cancer tissues)were detected and compared by polymerase chain reaction(PCR).The relationship between the expression of lncRNA HCG18,miR-152-3p and the clinicopathological features of ovarian cancer patients was analyzed.Ovarian cancer patients were followed up for 3 years,the 3 years cumulative survival rate in ovarian cancer patients with high expression and low expression of lncRNA HCG18 and miR-152-3p were compared by Kaplan-Meier survival curve.The prognostic factors of ovarian cancer patients were analyzed by Cox risk model was used to analyze.Results:Comparison of the expression levels of lncRNA HCG18,ovarian cancer tissues was higher than that of adjacent tissues(P＜0.05),and Comparison of the expression levels of miR-152-3p,ovarian cancer tissues was lower than that of adjacent tissues(P＜0.05).The expression levels of lncRNA HCG18 and miR-152-3p in ovarian cancer tissues were related to International Federation of Gynecology and Obstetrics(FIGO)stage,degree of differentiation,ascites and lymph node metastasis(P＜0.05).The 3 years cumulative survival rate in high lncRNA HCG18 group was 41.57％(37/89),which was lower than 70.65％(65/92)in low lncRNA HCG18 group,there was a difference of statistical significance(P＜0.05).The 3 years cumulative survival rate in high miR-152-3p group was 75.53％(71/94),which was higher than 35.63％(31/87)in low miR-152-3p group,there was a difference of statistical significance(P＜0.05).Cox proportional hazard model analysis showed that,FIGO stage Ⅲ～IV,high and moderate differentiation,ascites,lymph node metastasis,lncRNA HCG18 increased,miR-152-3p decreased were risk factors for poor prognosis in ovarian cancer patients.Conclusion:The high expression of lncRNA HCG18 and the low expression of miR-152-3p in ovarian cancer tissues are relate to the occurrence of malignant clinicopathological features and poor prognosis.
关键词
卵巢癌/lncRNA/HCG18/miR-152-3p/病理特征/预后Key words
Ovarian cancer/lncRNA HCG18/miR-152-3p/Pathological features/Prognosis引用本文复制引用
基金项目
珠海市社会发展领域科技计划项目(2320000040000287)
珠海市社会发展领域科技计划项目(22200040000198)
广东省自然科学基金面上项目(2023A1515010515)
出版年
2024
NETL
NSTL
万方数据