Study on the Relationship between Serum miR-191-5p,miR-101-3p and Clinicopathological Features,PI3K/Akt Signaling Pathway and Prognosis in Gastric Cancer Patients
Objective:To investigate the relationship between serum microribonucleic acid(miR)-191-5p,miR-101-3p and clinicopathological features,phosphatidylinositol 3 kinase(PI3K)/protein kinase B(Akt)signaling pathway and prognosis in gastric cancer patients.Methods:182 gastric cancer patients(gastric cancer group)admitted to our hospital from January 2019 to January 2021 and 90 healthy subjects(control group)who underwent physical examination in the physical examination center during the same period were selected.The expressions of serum miR-191-5p,miR-101-3p,PI3K mRNA and Akt mRNA were detected in two groups.The correlation between serum miR-191-5p,miR-101-3p and PI3K mRNA,Akt mRNA was analyzed by Pearson.The relationship between serum miR-191-5p,miR-101-3p and clinicopathological features of gastric cancer patients was analyzed.The 3-year overall survival(OS)rate of gastric cancer patients with different miR-191-5p and miR-101-3p expression was analyzed by Kaplan-Meier curve.Results:The expression of serum miR-191-5p and miR-101-3p in gastric cancer group was lower than that in control group(P＜0.05),and the expression of PI3K mRNA and Akt mRNA was higher than that in control group(P＜0.05).The expression of serum miR-191-5p and miR-101-3p in gastric cancer patients was negatively correlated with PI3K mRNA and Akt mRNA(P＜0.05).The expression of serum miR-191-5p and miR-101-3p was related to TNM stage,lymph node metastasis,depth of invasion and differentiation of gastric cancer(P＜0.05).The 3-year OS rate of gastric cancer patients with low expression of miR-191-5p was lower than that of gastric cancer patients with high expression of miR-191-5p,and the 3-year OS rate of gastric cancer patients with low expression of miR-101-3p was lower than that of gastric cancer patients with high expression of miR-101-3p(P＜0.05).Conclusion:The expression of serum miR-191-5p and miR-101-3p in gastric cancer patients are significantly down-regulate,which are relate to TNM stage,lymph node metastasis,depth of invasion and degree of differentiation,may lead to poor prognosis by activating PI3K/Akt signaling pathway.
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