Application of Different Histological Staining Methods in Preclinical Animal Trials of Polylactic Acid Facial Fillers
Objective:To explore the application value of HE staining,Masson staining,Sirius red staining and immunofluorescence staining in the animal experimental study of polylactic acid facial fillers in promoting collagen regeneration.Methods:Tissue specimens embedded with polylactic acid facial fillers obtained from animal experiments were selected for histological staining,and the reaction of surrounding tissues and the regeneration of type Ⅰ and Ⅲ collagen were observed under microscope.The applicability of different staining methods in the evaluation of collagen regeneration in animal experiments was compared.Results:HE staining can be used for the identification of infiltrating inflammatory cells,fibrosis and cystic formation,neovascularization and fat infiltration.Masson staining can be used to study the deposition and distribution of collagen fibers,but it can not be used to classify collagen.Sirius red staining can be used to study collagen neovascularization and distinguish type Ⅰ and type Ⅲ collagen fibers under polarized light microscope.Immunofluorescence staining can be specifically labeled to show type Ⅰ collagen and type Ⅲ collagen respectively,which can be used for the classification of collagen fibers.Conclusions:Combined with different staining methods,the type and number of inflammatory cells,collagen deposition,neovascularization,fat infiltration,fibrosis and other histomorphological changes can be studied.it can be used in animal experimental study on the safety and efficacy of medical facial fillers.
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