目的:通过实验观察健脾利湿通脉方对DVT大鼠miR-23a及相关炎性因子的影响。方法:将140只Wistar大鼠随机分为Blank group、Sham control、Modelset、Low dose group、Middle dose group、High-dose group 和 Positive control，每组 10 只，构建 DVT模型。分别于给药后7天、14天测定miR-23a、IL-1β、IL-6及TNF-α含量。结果:1。14d，与Blank group比较，Modelset大鼠TNF-α、IL-1β、IL-6、及 miR-23a 表达显著升高(P＜0。05);与 Modelset 比较，Low dose group、Middle dose group、High-dose group 及Positive control 大鼠上述指标均显著改善(P＜0。05);在 TNF-α 组别内，与 Positive control 比较，Blank group、Sham control、Modelset、Low dose group、High-dose group 均有显著性差异(P＜0。05);在 IL-1β 组别内，与 Positive control 比较，Sham control、Modelset、High-dose group 均有显著性差异(P＜0。05);在 IL-6 组别内，与 Positive control 比较，Blank group、Sham control、Middle dose group、High-dose group 均有显著性差异(P＜0。05);在 miR-23a 组别内，与 Positive control 比较，Blank group、Sham control、Modelset、Low dose group、Middle dose group 均有显著性差异(P＜0。05)。2。7 d 与 14 d 相比，在大鼠 IL-1β、IL-6、TNF-α 及miR-23a 四个组别中，Blank group、Sham control 前后无明显统计学差异(P＞0。05);而 Modelset、Low dose group、Middle dose group、High dose group、Positive control前后有明显统计学差异(P＜0。05)。结论:健脾利湿通脉方通过下调miR-23a及IL-1β、IL-6、TNF-α表达而发挥治疗作用。
The Effects of Jianpi-Lishi-Tongmai Decoction on miR-23a and Inflammatory Factors in Rats with Deep Vein Thrombosis
Objective:To experimentally observe the effects of Strengthening the Spleen,Promoting Dampness and Channeling the Veins Formula on miR-23a and related inflammatory factors in DVT rats.Methods:140 Wistar rats were randomly divided into Blank group,Sham control,Modelset,Low dose group,Middle dose group,High-dose group and Positive control,10 rats in each group,and DVT model was constructed.The contents of miR-23a,IL-1β,IL-6 and TNF-α were measured at 7 and 14 days after drug administration,respectively.Results:1.At 14 d,the expression of TNF-α,IL-1(3,IL-6,and miR-23a was signifiicantly elevated in Modelset rats compared with that in Blank group(P＜0.05);the expression of the above indexes was significantly elevated in Low dose group,Middle dose group,High-dose group,and Positive control rats compared with that in Modelset.Positive control rats showed significant improvement in the above indexes(P＜0.05);within the TNF-α group,Blank group,Sham control,Modelset,Low dose group,High-dose group showed significant differences compared with Positive control(P＜0.05);within the IL-1 β group,there was a significant difference between Sham control,Modelset,and High-dose group when compared with Positive control(P＜0.05);within the IL-6 group,Blank group when compared with Positive control,Sham control,Middle dose group,and High-dose group were all significantly different(P＜0.05);within the miR-23a group,compared with Positive control,Blank group,Sham control,Modelset,Low dose group,and Middle dose group were significantly different(P＜0.05).2.In the four groups of IL-1 β,IL-6,TNF-α,and miR-23a in rats at 7 d compared with 14 d,there were no statistically significant differences before and after the Blank group,and the Sham control(P＞0.05);and the Modelset,Low dose group,Middle dose group,High dose group,Positive control before and after had significant statistical differences(P＜0.05).Conclusion:Strengthening the spleen,inducing dampness and promoting circulation formula exerted therapeutic effects by down-regulating miR-23a and the expression of IL-1β,IL-6,and TNF-α.
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