纳-葡萄糖共转运蛋白2抑制剂调控TLRs/MyD88通路对高糖诱导HAECs自噬及凋亡的影响
The Effect of SGLT-2i Regulation of TLRs/MyD88 Pathway on High Glucose Induced Autophagy and Apoptosis in Human Aortic Endothelial Cells
曹新营 1邢彩耐 1刘丽丽 1杨光 1刘馨蕊1
作者信息
- 1. 华北理工大学附属医院心血管内科 河北唐山 063000
- 折叠
摘要
目的:探讨纳-葡萄糖共转运蛋白2抑制剂(SGLT-2i)调控TLRs/MyD88通路对高糖诱导的人主动脉内皮细胞(HAECs)自噬及凋亡的影响.方法:选用HAECs,将细胞分为NG组(5.5mmol/L葡萄糖)、HG组(30mmol/L葡萄糖)和SGLT-2i+HG组(30 mmol/L葡萄糖+50 μmol/L的SGLT-2抑制剂).检测出各细胞生长活力、细胞凋亡水平、细胞TLRs、MyD88 mRNA表达水平,细胞TLRs、MyD88、Bax、Bcl-2、Beclin 1、LC3Ⅱ蛋白表达以及自噬水平.结果:与NG组相比,HG组细胞增值率显著降低(P＜0.05);与HG组相比,SGLT-2i+HG组细胞增值率显著上升(P＜0.05).12h时24h时时间点,与NG组相比,HG组细胞凋亡率均显著升高(P＜0.05);与HG组相比,SGLT-2i+HG组细胞凋亡率均显著下降(P＜0.05).与NG组相比,HG组凋亡相关蛋白Bax、Bcl-2蛋白表达水平升高(P＜0.05);与HG组相比,SGLT-2i+HG组细胞Bax、Bcl-2表达水平下降(P＜0.05).与NG组相比,HG组自噬相关蛋白 Beclin 1、LC3Ⅱ 水平升高(P＜0.05);与 HG 组相比,SGLT-2i+HG 组细胞 Beclin 1、LC3Ⅱ 水平下降(P＜0.05).与 NG 组相比,HG组TLRs、MyD88蛋白表达水平升高(P＜0.05);与HG组相比,SGLT-2i+HG组细胞TLRs、MyD88表达水平下降(P＜0.05).结论:SGLT-2通过调控TLRsMyD88通路抑制高糖诱导的人主动脉内皮细胞自噬及凋亡.
Abstract
Objective:To investigate the effect of sodium glucose cotransporter 2 inhibitor(SGLT-2i)regulating the TLRs/MyD88 pathway on high glucose induced autophagy and apoptosis in human aortic endothelial cells(HAECs).Methods:Using HAECs,the cells were divided into NG group(5.5 mmol/L glucose),HG group(30 mmol/L glucose),and SGLT-2i+HG group(30 mmol/L glucose+50)μMol/L of SGLT-2 inhibitor.Detect the growth vitality;the level of cell apoptosis;the expression levels of TLRs and MyD88 mRNA;the levels of TLRs,MyD88,Bax,Bcl-2,Beclin 1,and LC3Ⅱ in each group of cells.Results:Compared with the NG group,the cell proliferation rate of the HG group was significantly reduced(P＜0.05);Compared with the HG group,the SGLT-2i+HG group showed a significant increase in cell proliferation rate(P＜0.05).At 12 hours and 24 hours,compared with the NG group,the apoptosis rate of cells in the HG group was significantly increased(P＜0.05);Compared with the HG group,the apoptosis rate of SGLT-2i+HG group was significantly reduced(P＜0.05).Compared with the NG group,the expression levels of apoptosis related proteins Bax and Bcl-2 in the HG group increased(P＜0.05);Compared with the HG group,the expression levels of Bax and Bcl-2 in cells of the SGLT-2i+HG group decreased(P＜0.05).Compared with the NG group,the levels of autophagy related proteins Beclin 1 and LC3Ⅱ in the HG group increased(P＜0.05);Compared with the HG group,the levels of Beclin 1 and LC3Ⅱ in cells of the SGLT-2i+HG group decreased(P＜0.05).Compared with the NG group,the expression levels of TLRs and MyD88 proteins in the HG group increased(P＜0.05);Compared with the HG group,the expression levels of TLRs and MyD88 in the SGLT-2i+HG group decreased(P＜0.05).Conclusion:SGLT-2 inhibits high glucose induced autophagy and apoptosis in human aortic endothelial cells by regulating the TLRsMyD88 pathway.
关键词
SGLT-2i/高糖/主动脉内皮细胞/自噬/凋亡/TLRs/MyD88通路Key words
SGLT-2i/High sugar/Aortic endothelial cells/Autophagy/Apoptosis/TLRs/MyD88 pathway引用本文复制引用
出版年
2024
NETL
NSTL
万方数据