The Effect of SGLT-2i Regulation of TLRs/MyD88 Pathway on High Glucose Induced Autophagy and Apoptosis in Human Aortic Endothelial Cells
Objective:To investigate the effect of sodium glucose cotransporter 2 inhibitor(SGLT-2i)regulating the TLRs/MyD88 pathway on high glucose induced autophagy and apoptosis in human aortic endothelial cells(HAECs).Methods:Using HAECs,the cells were divided into NG group(5.5 mmol/L glucose),HG group(30 mmol/L glucose),and SGLT-2i+HG group(30 mmol/L glucose+50)μMol/L of SGLT-2 inhibitor.Detect the growth vitality;the level of cell apoptosis;the expression levels of TLRs and MyD88 mRNA;the levels of TLRs,MyD88,Bax,Bcl-2,Beclin 1,and LC3Ⅱ in each group of cells.Results:Compared with the NG group,the cell proliferation rate of the HG group was significantly reduced(P＜0.05);Compared with the HG group,the SGLT-2i+HG group showed a significant increase in cell proliferation rate(P＜0.05).At 12 hours and 24 hours,compared with the NG group,the apoptosis rate of cells in the HG group was significantly increased(P＜0.05);Compared with the HG group,the apoptosis rate of SGLT-2i+HG group was significantly reduced(P＜0.05).Compared with the NG group,the expression levels of apoptosis related proteins Bax and Bcl-2 in the HG group increased(P＜0.05);Compared with the HG group,the expression levels of Bax and Bcl-2 in cells of the SGLT-2i+HG group decreased(P＜0.05).Compared with the NG group,the levels of autophagy related proteins Beclin 1 and LC3Ⅱ in the HG group increased(P＜0.05);Compared with the HG group,the levels of Beclin 1 and LC3Ⅱ in cells of the SGLT-2i+HG group decreased(P＜0.05).Compared with the NG group,the expression levels of TLRs and MyD88 proteins in the HG group increased(P＜0.05);Compared with the HG group,the expression levels of TLRs and MyD88 in the SGLT-2i+HG group decreased(P＜0.05).Conclusion:SGLT-2 inhibits high glucose induced autophagy and apoptosis in human aortic endothelial cells by regulating the TLRsMyD88 pathway.
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