摘要
目的:探讨p16蛋白与HPV-DNA、TCT联合应用在宫颈癌病变筛查的价值研究.方法:回顾性分析2023年02月至2023年12月在广州市某地区妇幼保健院就诊疑似患者3952例,并行p16蛋白检测与HPV-DNA筛查、液基薄层细胞(TCT)以及宫颈癌病理切片(HE)鉴定,分析、比较不同年龄段组女性p16蛋白免疫组化、HPV-DNA分型以及TCT检测结果相关性.结果:p16蛋白检测阳性率随着HPV-DNA筛查增高有递增趋势(差异具有统计学意义,P＜0.05).p16蛋白检测随着TCT诊断结果分级NILM与ASC-US、ASC-H、LSIL、HSIL、SCC组别的升高有递增趋势(差异具有统计学意义,P＜0.05).p16蛋白检测随着宫颈癌病理切片HE染色分级增高有递增趋势(差异具有统计学意义,P＜0.05).p16蛋白检测阳性率与患者年龄关系密切;在3952例妇女中HPV-DNA筛查阳性患者1836例,阳性率约46.46％;TCT检测结果阳性有1528名,阳性率约38.66％;按年龄分组结果:发病年龄30岁以后有上升趋势,41-50岁是发病高峰年龄段,60岁以后发病率下降,年龄呈抛物线向上及下趋势;宫颈癌发病还有呈年轻化趋势.结论:p16蛋白与HPV-DNA、TCT联合应用提高了宫颈癌及其癌前病变筛查的准确度.
Abstract
Objective:To investigate the effect p16 protein combined with HPV-DNA and TCT in screening cervical cancer lesions.Methods:A retrospective analysis was conducted on 3952 suspected patients who visited a women's and children's health hospital in a certain area of Guangzhou from February 2023 to December 2023.Parallel p16 protein detection,HPV-DNA screening,Thin-prep cytologic test(TCT),and cervical cancer histopathological section(HE)identification were performed.The correlation between p16 protein immunohistochemistry,HPV-DNA typing,and TCT test results in different age groups of women was analyzed and compared.Results:The positive rate of p1 6 protein detection tended to increase with the increase in HPV-DNA screening(the difference was statistically significant,P＜0.05).p16 protein detection tended to increase with the grading of TCT diagnosis results from NILM to ASC-US,ASC-H,LSIL,HSIL,and SCC groups(the difference was statistically significant,P＜0.05).p16 protein detection tended to increase with the grading of cervical cancer histopathological sections by HE staining(the difference was statistically significant,P＜0.05).The positive rate of p16 protein detection was closely related to patient age;among the 3952 women,1836 were HPV-DNA screening positive,with a positive rate of approximately 46.46％;1528 were TCT positive,with a positive rate of approximately 38.66％;grouped by age,the onset age tended to increase after 30 years old,peaked at 41-50 years old,and decreased after 60 years old,showed an upward and downward parabolic trend with age;the onset of cervical cancer also showed a trend of younger age.Conclusion:The combination of p16 protein,HPV-DNA,and TCT improved the accuracy of cervical cancer and precancerous lesion screening.
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