Early Alterations of Neuronal Activity in the Visual Cortex of APP/PS1 Mouse Model of Alzheimer's Disease
Objective:Investigate the changes of neuronal activity in the primary visual cortex of 2-month-old APP/PS1 mice,an Alzheimer's disease model,under different visual stimulation conditions,and elucidate the early functional alterations in the visual cortex of APP/PS1 mice.Methods:The expression of Aβ in the primary visual cortex of 2-month-old APP/PS1 mice was analyzed by im-munofluorescence staining,and 2-month-old APP/PS1 and wild type(WT)mice were selected and subjected to different visual stimula-tion conditions.The changes of neuronal activity in the primary visual cortex were analyzed by performing immunofluorescent staining.Results:Aβ is expressed in V1 of 2-month-old APP/PS1 mice,but no obvious amyloid plaques has been found.In the absence of external visual stimulation,the c-Fos positive rate in the V1 of 2-month-old APP/PS1 mice is higher than that in wild-type mice(P＜0.01).When given 30 minutes of visual stimulation to mice or 90 minutes after visual stimulation,there is no significant difference in c-Fos positive rate in the V1 of 2-month-old APP/PS1 and WT mice in both cases.Conclusions:The neurons in the V1 of 2-month-old APP/PS1 mice are in a hyperactive state in the absence of external visual stimulation,suggesting altered activity patterns of cortical neurons in the visual cortex of young APP/PS1 mice.
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