The Mechanism of UC Intervention on PKC-NF-κB Pathway in the Treatment of Ulcerative Colitis
Objective:To investigate the mechanism of UC intervention on PKC-NF-κB pathway in the treatment of ulcerative colitis.Methods:In this study,33 adult male SD rats were selected and divided into five groups according to the research requirements,they were divided into normal group,low-dose group,middle-dose group,high-dose group and UC Group,among which,normal group(N=7),high-dose group(N=6),middle-dose group(N=6),low-dose group(N=9)and UC Group(N=5).The immunohistochemical re-sults,colonic results and hypothalamic results were analyzed.The relative expression levels of cytokines in colon tissue were measured to confirm the mechanism of UC intervention on PKC-NF-κB pathway in the treatment of ulcerative colitis.Results:The results of immuno-histochemistry showed that there were significant differences between UC Group and high-dose group,middle-dose group,low-dose group and normal group(P＜0.05),there were significant differences in the results of colonic examination and hypothalamus examination among the five groups(P＜0.05),the indexes of UC Group,high dose group,middle dose group and low dose group were higher than those of normal group(P＜0.05).The indexes of high dose group were higher than those of middle dose group,low dose group and normal group(P＜0.05),the level in the middle dose group was higher than that in the low dose group and the normal group(P＜0.05),and the level in the low dose group was higher than that in the normal group(P＜0.05).Conclusion:UC intervention of PKC-NF-κB pathway is effective in the treatment of ulcerative colitis,and can be directly related to the relative expression level of cytokine mRNA in colon tis-sue,UC intervention can therefore help in the treatment of ulcerative colitis.
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