儿童阻塞性睡眠呼吸暂停患者外周血白细胞组学分析
Peripheral Blood Leukocyte Profiling in Children with Obstructive Sleep Apnea
齐雨薇 1孔雅茹 2季洁 3王珊 4邰隽5
作者信息
- 1. 首都儿科研究所 北京 100020
- 2. 首都儿科研究所 北京 100020;北京协和医学院研究生院首都儿科研究所 北京 100020
- 3. 首都医科大学附属北京儿童医院 北京 100045
- 4. 首都儿科研究所 北京 100020;首都儿科研究所儿童发育营养组学北京市重点实验室 北京 100020
- 5. 首都儿科研究所 北京 100020;首都儿科研究所附属儿童医院耳鼻咽喉头颈外科 北京 100020
- 折叠
摘要
目的:研究儿童阻塞性睡眠呼吸暂停患者与健康儿童的外周血白细胞在基因表达和功能富集方面的差异情况.方法:招募我院确诊为阻塞性睡眠呼吸暂停(OSA)的患儿5名作为病例组,以及健康儿童7名作为对照组.多导睡眠监测结束后的清晨采集静脉血3 mL,离心分离白细胞并提取RNA,进行高通量测序和转录组学分析,鉴定差异表达基因,并进行GO项目和KEGG项目通路富集、蛋白互作分析.结果:在比较OSA患儿与健康组的外周血白细胞时,发现两者在基因表达水平上有显著差异,这些差异主要集中在与炎症反应和缺氧相关的生物过程.显著改变的信号通路涵盖了细胞因子与细胞因子受体的相互作用、Toll样受体信号传导以及MAPK信号传导等.此外,一些关键蛋白的相互作用也受到影响,包括细胞周期调控蛋白(如CDK1、CCNB1、BUB1)、干扰素诱导蛋白(如IFIT1、XAF1)以及多种细胞因子(如CXCL8、IL6、TNF).结论:OSA患儿的外周血白细胞的免疫和炎症相关基因表达变化显著,提示存在全身炎症反应,这可能是儿童OSA发展的潜在机制.
Abstract
Objective:To study the differences of gene expression and functional enrichment of peripheral blood leukocytes be-tween pediatric patients with obstructive sleep apnea(OSA)and healthy children.Methods:5 children diagnosed with OSA were selected as the case group and 7 healthy children were selected as the control group.Venous blood(3mL)was collected in the morning after polysomnography monitoring.White blood cells were isolated by centrifuge and then RNA was extracted for high-throughput sequencing and transcriptome analysis.Differentially expressed genes were identifiied,followed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment,as well as protein-protein interaction analysis.Results:There were significant differences in the gene expression levels of peripheral blood leukocytes between the case group and the control group,mainly in the biological processes related to inflammatory response and hypoxia.The signaling pathways involved include the interaction between cytokines and their receptors,Toll-like receptor signaling pathway and MAPK signaling pathway.Key interacting proteins involved include cell cycle regulatory pro-teins(e.g.CDK1,CCNB1,BUB1),interferon-inducing proteins(e.g.IFIT1,XAF1),and various cytokines(e.g.CXCL8,IL6,TNF).Con-clusions:The expression of immune and inflammation-related genes in peripheral white blood cells of children is significantly changed,suggesting systemic inflammation,which may be a potential mechanism of OSA in children.
关键词
儿童/阻塞性睡眠呼吸暂停/炎症/转录组学Key words
Children/Obstructive sleep apnea/Inflammation/Transcriptomics引用本文复制引用
出版年
2024
NETL
NSTL
万方数据