Peripheral Blood Leukocyte Profiling in Children with Obstructive Sleep Apnea
Objective:To study the differences of gene expression and functional enrichment of peripheral blood leukocytes be-tween pediatric patients with obstructive sleep apnea(OSA)and healthy children.Methods:5 children diagnosed with OSA were selected as the case group and 7 healthy children were selected as the control group.Venous blood(3mL)was collected in the morning after polysomnography monitoring.White blood cells were isolated by centrifuge and then RNA was extracted for high-throughput sequencing and transcriptome analysis.Differentially expressed genes were identifiied,followed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment,as well as protein-protein interaction analysis.Results:There were significant differences in the gene expression levels of peripheral blood leukocytes between the case group and the control group,mainly in the biological processes related to inflammatory response and hypoxia.The signaling pathways involved include the interaction between cytokines and their receptors,Toll-like receptor signaling pathway and MAPK signaling pathway.Key interacting proteins involved include cell cycle regulatory pro-teins(e.g.CDK1,CCNB1,BUB1),interferon-inducing proteins(e.g.IFIT1,XAF1),and various cytokines(e.g.CXCL8,IL6,TNF).Con-clusions:The expression of immune and inflammation-related genes in peripheral white blood cells of children is significantly changed,suggesting systemic inflammation,which may be a potential mechanism of OSA in children.
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