Study on the Relationship between the Expression of miR-205 and CHN1 in Cervical Cancer Tissues and High-Risk HPV Infection and Prognosis
Objective:To study the relationship between the expression of microRNA(miR)-205 and α-chimeric protein(CHN1)in cervical cancer(CC)tissues and high-risk human papillomavirus(HPV)infection and prognosis.Methods:182 patients with CC admitted to Xuzhou Municipal Hospital Affiliated to Xuzhou Medical University from January 2018 to February 2021 were selected as CC group,and 150 patients with cervical intraepithelial neoplasia(CIN)admitted to Xuzhou Municipal Hospital Affiliated to Xuzhou Medical University during the same period were selected as CIN group and 150 patients with cervicitis were selected as cervicitis group.The high expression rate of CHN1,the relative expression of miR-205 and the positive infection rate of high-risk HPV were compared among three groups.The high expression rate of CHN1 and the relative expression of miR-205 in CC patients with different high-risk HPV infection types and viral load were compared.The high expression rates of CHN1 and miR-205 in CC patients with different clinicopathological features were compared.The correlation between the expression of CHN1 and miR-205 and high-risk HPV infection in CC patients were analyzed by Spearman test.CC patients were followed up for 3 years,the 3-year overall survival rate of CC patients in CHN1 and miR-205 low and high expression groups were analyzed by Kaplan-Meier method and Log-Rank test.Results:The high expression rate of CHN1,the relative expression of miR-205 and the positive infection rate of high-risk HPV in CC group were higher than those in CIN group and cervicitis group,and the high expression rate of CHN1,the relative expression of miR-205 and the positive infection rate of high-risk HPV in CIN group were higher than those in cervicitis group(P＜0.05).Among 166 CC patients with high-risk HPV positive in-fection,the higher the viral load,the higher the CHN1 high expression rate and the higher the relative expression of miR-205,and the high expression rate of CHN1 and the relative expression of miR-205 in multiple infection were significantly higher than those in single infection(P＜0.05).There was no statistical difference in the high expression rate of CHN1 and miR-205 among CC patients with different age,tumor diameter,pathological type and differentiation degree(P＞0.05),the high expression rate of CHN1 and miR-205 in CC patients with lymph node metastasis and FIGO stage ⅡA were significantly higher than those in CC patients without lymph node metastasis and FIGO stage Ⅰ A～Ⅰ B(P＜0.05).Spearman test showed that,the high expression of CHN1 and miR-205 were positively correlated with high-risk HPV infection in CC patients(P＜0.05).3 years after follow-up,3 of 182 CC patients were lost to follow-up,and the 3-year over-all survival rate was 78.21％.Compared with low expression group of CHN1 and miR-205,the 3-year overall survival rate in high ex-pression group of CHN1 and miR-205 was significantly lower(P＜0.05).Conclusion:The high expression of CHN1 and miR-205 in CC tissues is related to the occurrence of high-risk HPV infection and the decrease of overall survival rate,which may synergistically regulate high-risk HPV infection to affect the occurrence,development and prognosis of CC.
Cervical cancerMicroRNA-205α-chimeric proteinHigh-risk human papilloma virusInfectionPrognosis
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