Analysis of Water Quality Status and Microbial Community Structure in Four Functional Water Bodies
Objective:To investigate the water quality and its microbial community structure in four different functional water bodies-reservoirs,the South-to-North Water Diversion East Route water,seawater bathing beaches,and seawater aquaculture areas.Methods:Sampling was carried out in each of the four functional areas of water bodies.Through 16S rRNA high-throughput sequencing technology,combined with the physical and chemical indicators of water quality,the current status of water quality as well as the differ-ences in the structural composition of microbial communities in different functional water bodies were analysed and compared.Results:The water quality of the sea bathing beach was the best compared to the other three water body functional areas,TN was high in the reservoir,COD and TN were higher in the water of the east line of the South-to-North Water Diversion,and the concentration of reactive phosphate was slightly higher in the sea water aquaculture area.Species richness as well as species diversity were highest in the four wa-ter body functional areas in the sea bathing area,and lower in the reservoir and mariculture area.Differences in bacterial community composition were mainly at the genus level.Reservoir and South-to-North Water Diversion East Line water compositions were similar compared to the other two functional areas,mainly due to the fact that the winter water supply of the Echinohontan Reservoir only comes from the South-to-North Water Diversion East Line water.In addition there were differences in the bacterial composition between the sea bathing area and the sea water aquaculture area,and aquaculture and the consequent changes in the physicochemical factors of the water quality had a greater impact on the differences in the composition of the bacterial communities in the two areas.Total nitrogen and total phosphorus were the main environmental factors affecting the bacterial community composition.Conclusions:The water quality of the sea bathing beaches is the most compliant with the standards,while the high TN in the reservoirs and the water of the South-to-North Water Diversion Eastern Route increases the risk of eutrophication of the water bodies and needs to be focused on.The source of im-poundment,high TN,COD and reactive phosphate concentrations have a greater impact on causing differences in the bacterial community composition of the four functional water bodies.
Water functional zonesHigh-throughput sequencingEnvironmental factorsBacterial community
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