Effect of LncRNA SNHG1 on the Damage of Alzheimer's Disease Cell Models through the miR-361-3p/Nfat5 Signaling Pathway
Objective:To investigate the effect of LncRNA SNHG1 expression on AD cell damage model,and to study its rela-tionship with miR-361-3p/Nfat5 pathway.Methods:Establish an AD cell model.To SH-SY 5 Y cells to SNHG 1-shRNA to reduce SNHG 1 expression and to miR-361-3p-mimic to overexpress miR-361-3p.The expression levels of SNHG 1 and miR-361,apoptosis,and protein expression were examined,and miR-361-3p was bound to SNHG 1.Results:Compared with the blank control group,the AD model group showed decreased miR-361-3p expression,but increased SNHG 1 expression and increased apoptosis rate(P＜0.05).Knock-down of reduced SNHG 1 expression decreased the apoptosis rate(P＜0.05).Overexpression of miR-361-3p reduces the level of SNHG 1 expression.In the AD model group,Nfat 5 and p-Tau were increased,while Nestin protein decreased(P＜0.05);knockdown of SNHG 1 decreased the expression of Nfat 5 and p-Tau and increased the Nestin protein expression.Conclusion:Knockdown of SNHG1 can re-duce the damage induced by Aβ25-35 protein in AD cell models,and the mechanism is related to the activation of miR-361-3p/Nfat5 pathway.
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