Effects of Duhuo Jisheng Decoction Combined with Spot Warming Acupuncture and Moxibustion at Jiejin Focus on Oxidative Stress,Cartilage Metabolic Indicators and NLRP3 Inflammatory Bodies in Patients with Knee Osteoarthritis
Objective:To analyze the effects of duhuo jisheng decoction combined with needle warming moxibustion on oxidative stress,cartilage metabolism indexes and nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3)inflammasome in patients with knee osteoarthritis(KOA).Methods:The random number table method was used,135 patients with KOA of cold-dampness obstruction type were divided into control group(67 cases,treated with warm acupuncture at the lesion point of the tendon),and study group(68 cases,treated with duhuo jisheng decoction combined with warm acupuncture at the lesion point of the tendon).The Lysholm knee score,western ontario and mcMaster universities osteoarthritis Index(WOMAC),serum oxidative stress indexes[superoxide dismu-tase(SOD),malondialdehyde(MDA)],serum cartilage metabolism indexes[type Ⅱ collagen carboxy terminal peptide(CTX-Ⅱ),proteo-glycan(PG),cartilage oligomeric matrix protein(COMP)],and the expression of NLPR3 inflammasome mRNA in peripheral blood mononuclear cells were compared between two groups.Results:Lysholm knee score and serum SOD increased in two groups,and study group was higher than those in control group after treatment.WOMAC score,serum MDA,COMP,CTX-Ⅱ,PG,NLRP3 mRNA expres-sion,apoptosis-related spot-like protein(ASC)mRNA expression,and caspase-1 mRNA expression decreased,and study group was lower than those in control group(P＜0.05).Conclusion:Duhuo jisheng decoction combined with warm acupuncture and moxibustion at the focus of tendon knots in the treatment of KOA patients,which can reduce oxidative stress,improve cartilage metabolism indexes,inhibit NLRP3 inflammasome.
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