Research on the Relationship between Serum lncRNA NNT-AS1 and miR-582-5p and Airway Inflammation and Airway Hyperresponsiveness in Children with Bronchial Asthma
Objective:To investigate the relationship between serum long non-coding RNA(lncRNA)nicotinamide nucleotide antisense transferase RNA1(NNT-AS1),microRNA(miR)-582-5p and airway inflammation and airway hyperresponsiveness in children with bronchial asthma.Methods:120 children with bronchial asthma were divided into remission group(n=52)and attack group(n=68)according to the relevant guidelines.Another 60 healthy children in the same period were selected as the control group.The relative expression of serum lncRNA NNT-AS1,miR-582-5p and serum airway inflammatory cytokines[interleukin-17(IL-17),tumor necrosis factor-α(TNF-α)],airway responsiveness indicators[basic respiratory resistance(Rrs cont),minimum induced cumulative dose(Dmin),basic respiratory conductivity(Grs cont)]were compared among three groups.The relationship between serum lncRNA NNT-AS1,miR-582-5p expression and airway inflammatory cytokines,airway responsiveness indicators were analyzed by Pearson correlation test.Results:The relative expression of lncRNA NNT-AS1,serum levels of IL-17,TNF-α and Rrs cont in remission group and attack group were higher than those in control group,and those in attack group were higher than those in remission group(P＜0.05).The relative expression of miR-582-5p,Grs cont and Dmin in remission group and attack group were lower than those in control group,and those in attack group were lower than those in remission group(P＜0.05).The expression of serum lncRNA NNT-AS1 in bronchial asthma chil-dren was positively correlated with IL-7,TNF-α and Rrs cont(r＞0,P＜0.05),and negatively correlated with Grs cont and Dmin(r＜0,P＜0.05).The expression of serum miR-582-5p was negatively correlated with IL-17,TNF-α and Rrs cont(r＜0,P＜0.05),and positively corre-lated with Grs cont and Dmin(r＞0,P＜0.05).Conclusion:Serum lncRNA NNT-AS1 is highly expressed and miR-582-5p is lowly expressed in children with bronchial asthma,the expression of both are closely related to asthma stage,airway inflammation and airway hyperresponsiveness.
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