Mechanism of Arsenic Trioxide on Apoptosis and Autophagy in Triple-negative Breast Cancer Stem Cell MDA-MB-231 Cell Line
Objective:To investigate the mechanism of arsenic trioxide on apoptosis and autophagy in triple negative breast cancer(TNBC)stem cell MDA-MB-231 cell line.Methods:The stem cells cultured in the second generation were taken for 5 and divided into four groups:control group,arsenic trioxide acting group,Rapa combined with arsenic trioxide group and 3-MA combined with arsenic trioxide group.The apoptosis rate,autophagy and protein expression were observed.Results:For 48 h,the total apoptosis was 40％.When 3MA inhibited the autophagic activity,the apoptosis induced by arsenic trioxide decreased by about 50％,and Rapa promoted the arsenic trioxide induced apoptosis increased by about 50％.Compared with the control group,the intracellular fluorescence intensity was higher in arsenic trioxide,Rapa combined with arsenic trioxide,and 3-MA combined with arsenic trioxide.Bax,Beclin-1 and P62 protein expression were higher in 3-MA and arsenic trioxide group and Rapa group,and Bcl-2 protein expression was lower than the group and Rapa with arsenic trioxide group(P＜0.05).Conclusion:Arsenic trioxide induced autophagy and cell apoptosis coexist in MDA-MB-231 stem cells and may be regulated by common genes.Arsenic trioxide induced autophagy can promote cell apoptosis.
Triple-negative breast cancerArsenic trioxideApoptosisAutophagy
万方数据
维普
