Study of Acid-fast Staining,PCR-reverse Dot Blot Hybridization,PCR-fluorescent Probe Method in the Diagnosis of Mycobacterium Tuberculosis
Objective:To compare the value of acid-fast staining,PCR-reverse dot blot hybridization and PCR-fluorescent probe in the diagnosis of Mycobacterium tuberculosis infection.Methods:Samples from the Eighth Medical Center of the PLA General Hospital from January 2024 to April 2024 were selected,including suspected pulmonary tuberculosis and suspected extrapulmonary tuberculosis.Eighty samples were prepared into paraffin samples and tested for Mycobacterium tuberculosis by acid-fast staining,PCR-reverse dot blot hybridization and PCR-fluorescent probe.Results:The detection rates of PCR-reverse dot blot hybridization were 63.6％,PCR-fluorescent probe 59.1％,and acid-fast staining 25.0％.The detection rates of PCR-reverse dot blot hybridization and PCR-fluorescent probe were higher than those of acid-fast staining,and P＜0.05.The positive detection rate of PCR-reverse dot blot hybridization method and PCR-fluorescent probe method was basically the same.The acid-fast staining method has the characteristics of low detection cost and short experimental time,but it cannot clearly determine whether it is Mycobacterium tuberculosis infection based on morphology alone.The detection cost of PCR-reverse dot blot hybridization method was higher than that of acid-fast staining method,and the experimental time was longer than that of acid-fast staining method and PCR-fluorescent probe method.The PCR-fluorescent probe method is moderately expensive,but the detection time is shorter than that of PCR-reverse dot blot hybridization.Conclusions:The PCR-fluorescent probe method has a high detection rate,fast and simple operation,and moderate cost,which has good clinical application value for hospitals with a large number of samples.
Mycobacterium tuberculosisAcid-fast stainingPCR-reverse dot blot hybridizationPCR-fluorescent probePolymerase chain reaction
万方数据
维普
