Study on the Relationship between Serum CXCL10,CXCR4 Expression Levels and TLR4/NF-κB/NLRP3 Signaling Pathway and Lung Injury in Patients with Severe Acute Pancreatitis
Objective:To investigate the relationship between the expression levels of serum CXC chemokine ligand 10(CXCL10)and CXC chemokine receptor 4(CXCR4)and Toll-like receptor 4(TLR4)/nuclear factor-κB(NF-κB)/NOD-like receptor hot protein domain-related protein 3(NLRP3)signaling pathway and concurrent lung injury in patients with severe acute pancreatitis(SAP).Methods:157 patients with SAP were divided into damage group(57 cases)and undamaged group(100 cases)according to whether were complicated with lung injury,serum CXCL10,CXCR4 levels,TLR4,NF-κB,and NLRP3 messenger ribonucleic acid(mRNA)expression in peripheral blood mononuclear cells were detected and compared in two groups.The correlation between serum CXCL10,CXCR4 levels and TLR4,NF-κB,NLRP3 mRNA expression in peripheral blood mononuclear cells of SAP patients were analyzed by Pearson method.The influencing factors of SAP patients complicated with lung injury were analyzed by multivariate Logistic regression analysis.Results:The levels of serum CXCL10 and CXCR4 and the expression of TLR4 mRNA,NF-κB mRNA and NLRP3 mRNA in peripheral blood mononuclear cells in damage group were higher than those in undamaged group(P＜0.05).The levels of serum CXCL10 and CXCR4 in SAP patients were positively correlated with the expression of TLR4 mRNA,NF-κB mRNA and NLRP3 mRNA in peripheral blood mononuclear cells(P＜0.05).Elevated bedside severity index of acute pancreatitis(BISAP)score,hypoproteinemia,and elevated serum CXCL10 and CXCR4 levels were risk factors for lung injury in SAP patients(P＜0.05).Conclusion:The levels of serum CXCL10 and CXCR4 in SAP patients are increased,which are closely related to the activation of TLR4/NF-κB/NLRP3 signaling pathway and complicated with lung injury.
Severe acute pancreatitisLung injuryCXCL10CXCR4TLR4/NF-κB/NLRP3 signaling pathway
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