Effects of Dexmedetomidine Combined with Pulmonary Surfactant on Lung Injury and Hemodynamics in Rats with Lipopolysaccharide-Induced Acute Respiratory Distress Syndrome
Objective:To investigate the effects of dexmedetomidine(Dex)combined with pulmonary surfactant(PS)on lung in-jury and hemodynamics in rats with lipopolysaccharide induced acute respiratory distress syndrome(ARDS).Methods:50 SD rats were randomly divided into Control group,Model group,Dex group,PS group and Dex+PS group.The rats in control group were injected with normal saline,and the rats in other groups were established with ARDS model,Dex group,PS group and Dex+PS group were given Dex,PS and Dex combined with PS respectively,and the rats in other groups were perfused with normal saline.The hemodynamic changes in rats were detected by Doppler ultrasound.Lung function in rats was detected.Oxygen partial pressure(PaO2),carbon dioxide partial pressure(PaCO2)were detected by blood gas analyzer and the oxygenation index(OI)was calculated.The levels of tumor necrosis factor-α(TNF-α),interleukin(IL)-6 and IL-1β in bronchoalveolar lavage fluid were detected by enzyme-linked immunosorbent assay.Results:Compared with Control group,peak expiratory flow(PEF),forced vital capacity(FVC),forceful expiratory volume in 0.3 sec-onds(FEV0.3)/FVC levels,left ventricular systolic pressure(LVSP),heart rate(HR),mean arterial pressure(MAP),PaO2 and OI levels decreased in Model group.And PaCO2 level,TNF-α,IL-6 and IL-1β levels in alveolar lavage fluid increased(P＜0.05).Compared with Model group,PEF,FVC,FEV0.3/FVC levels,LVSP,MAP,HR,PaO2 and OI levels were elevated in Dex,PS,and Dex+PS groups,and Dex+PS group were higher than those of Dex and PS groups(P＜0.05).PaCO2,TNF-α,IL-6 and IL-1β levels in alveolar lavage fluid were decreased,and Dex+PS group was lower than that of Dex and PS groups(P＜0.05).Conclusion:The combined treatment of Dex and PS can alleviate inflammation and lung tissue injury,improve hemodynamics and lung function in ARDS rats.
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