Over-expression of AGM3,a Foreign Mutant Gene of AGAMOUS, Represses Flowering and Alters Floral Organ Development in Transgenic Tobacco
In order to investigate floral inhibition through over-expression of AGM3 in heterologous plants,a dominant negative mutation construct gene, 35S-AGM3-E9 was transformed into Nicotiana tabacum via the Agrobacterium-mediated method. The results of PCR and Southern analysis showed that the gene of AGM3 was integrated into tobacco genome. Furthermore, the data of real-time qRT-PCR analysis showed that AGM3 was expressed in all the transgenic lines and the transcripts of AGM3 were significantly different among these transgenic lines. The results of the investigation indicated that 46. 7％ of the transgenic lines did not flower during their life cycles, and flowering time of the 33. 3％ lines was delayed 29. 7 days averagely, while the rest 20％ of transgenic lines flowered at the same time with the wild type control. In addition, over-expression of AGM3 in transgenic tobacco plants caused decreased number of sepals, deep clefts on corolla, morphological changes of petals and stamens, and numerical change of stamens. The facts suggested that over-expression of AGM3 effectively suppress floral development, which laid a foundation for genetically engineered sterility.
AGM3Dominant negative mutationRepresses floweringFloral organ developmentTobacco
NETL
NSTL
万方数据
维普
