Cloning and Activity Analysis of Soybean FAD2-1B Promoter
The soybean oleate desaturase gene(FAD2-1B) has previously been shown to be expressed specifically in soybean seeds. The 5'-flanking upstream sequence of FAD2-1B gene, named FP, was isolated from the genomic DNA of soybean by PCR method. Sequence analysis by PLACE revealed that this fragment contains a series of motifs related to seed-specific promoters, such as Skn-1 motif,AACACA,SEF4 motif,E-box, ACGT. Replacing CaMV35S promoter of pCAMBIA1301 with FP fragment, the binary expression vector pCAM-FP was constructed. Transient expression in soybean tissues by Agrobacterium tumefaciens mediated method, the results of histochemical GUS analysis showed that there were little or not GUS activities in roots, stems and leaves, but there was higher activity in soybean seeds. It is inferred that FP promoter possess the function driven downstream gene expression exclusively in soybean seeds.
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万方数据
维普
